Optical clearing of the skin for near-infrared fluorescence image-guided surgery

• Published in: Journal of Biomedical Optics Vol. 14; no. 2; p. 024019
• Main Authors: Matsui, Aya, Lomnes, Stephen J, Frangioni, John V
• Format: Journal Article
• Language: English
• Published: United States 01.03.2009
• Subjects: Animals; Biomedical materials; Clearing; Dermatologic Surgical Procedures; Female; Fluorescence; Glycerol - administration & dosage; hyperosmotic agents; Image Enhancement - methods; image-guided surgery; Imaging; In vivo tests; Microscopy, Fluorescence - methods; near-infrared fluorescence; optical clearing; Polyethylene Glycols - administration & dosage; Skin - cytology; Skin - drug effects; Spectroscopy, Near-Infrared - methods; Surgery; Surgery, Computer-Assisted - methods; Surgical implants; Swine

Near-infrared (NIR) light penetrates relatively deep into skin, but its usefulness for biomedical imaging is constrained by high scattering of living tissue. Previous studies have suggested that treatment with hyperosmotic "clearing" agents might change the optical properties of tissue, re...