Inducing Cre-lox Recombination in Mouse Cerebral Cortex Through In Utero Electroporation

• Published in: Journal of visualized experiments no. 129
• Main Authors: Bland, Katherine M, Casey, Zachary O, Handwerk, Christopher J, Holley, Z Logan, Vidal, George S
• Format: Journal Article
• Language: English
• Published: United States MyJove Corporation 17.11.2017
• Subjects: Neuroscience

Cell-autonomous neuronal functions of genes can be revealed by causing loss or gain of function of a gene in a small and sparse population of neurons. To do so requires generating a mosaic in which neurons with loss or gain of function of a gene are surrounded by genetically unperturbed tissue. Here...