Dopaminergic neurotoxins require excitotoxic stimulation in organotypic cultures

• Published in: Neurobiology of disease Vol. 20; no. 3; pp. 639 - 645
• Main Authors: Kress, Geraldine J., Reynolds, Ian J.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.12.2005; Elsevier
• Subjects: 1-Methyl-4-phenylpyridinium - pharmacology; Animals; Animals, Newborn; Brain - metabolism; Brain - physiopathology; Cell Death - drug effects; Cell Death - physiology; Cell Nucleus - drug effects; Cell Nucleus - pathology; Cerebral Cortex - metabolism; Cerebral Cortex - physiopathology; Corpus Striatum - metabolism; Corpus Striatum - physiopathology; Dopamine - metabolism; Drug Synergism; Excitotoxicity; Glutamic Acid - metabolism; N-Methylaspartate - pharmacology; Nerve Degeneration - chemically induced; Nerve Degeneration - metabolism; Nerve Degeneration - physiopathology; Neurons - drug effects; Neurons - metabolism; Neurons - pathology; Neurotoxins - metabolism; Neurotoxins - pharmacology; NMDA receptors; Organ Culture Techniques; Organotypic culture; Oxidopamine - pharmacology; Parkinson Disease - metabolism; Parkinson Disease - physiopathology; Parkinson's disease; Rats; Rats, Sprague-Dawley; Rotenone - pharmacology; Substantia nigra; Substantia Nigra - metabolism; Substantia Nigra - physiopathology; Tyrosine 3-Monooxygenase - metabolism; Tyrosine hydroxylase; Tyrosine hydroxylase; Parkinson's disease; NMDA receptors; Excitotoxicity; Substantia nigra; Organotypic culture

We have investigated the properties of the dopaminergic neurotoxins 6-hydroxydopamine, 1-methyl-4-phenylpyridinium and rotenone using an organotypic culture that included slices of substantia nigra, striatum and cortex maintained for about 20 days in vitro. At this age, the organotypic culture conta...