The impact of skin viability on drug metabolism and permeation—BSA toxicity on primary keratinocytes

• Published in: Toxicology in vitro Vol. 20; no. 3; pp. 347 - 354
• Main Authors: Haberland, A., Schreiber, S., Maia, C. Santos, Rübbelke, M.K., Schaller, M., Korting, H.C., Kleuser, B., Schimke, I., Schäfer-Korting, M.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.04.2006
• Subjects: Albumin effects; Annexin A5 - metabolism; Apoptosis; Caspase 8; Caspase Inhibitors; Cell Survival - physiology; Cells, Cultured; Culture Media; Enzyme Inhibitors - pharmacology; Epidermis - cytology; Epidermis - drug effects; Epidermis - metabolism; Fluorescent Dyes; Glucocorticoids - metabolism; Human skin models; Humans; Indoles; Keratinocyte culture; Keratinocytes - drug effects; L-Lactate Dehydrogenase - metabolism; Metabolism; Permeability; Pharmaceutical Preparations - metabolism; Propidium - toxicity; Serum Albumin, Bovine - toxicity; Skin - metabolism; Skin Absorption; Skin viability; Skin viability; DMSO; Albumin effects; MTT; TNF; BSA; LDH; SD; DMEM; P21EC; MEME; KBM; PBS; SDS; TRAIL; Human skin models; DAPI; P17EC; Metabolism; Keratinocyte culture; KGM; PC; PD; FCS; PI; Apoptosis

For testing cutaneous absorption of drugs, ingredients of cosmetics and also for risk assessment of industrial compounds predictable in vitro test protocols are under investigation using excised skin or reconstructed human epidermis. Since the metabolizing enzymes expressed by viable skin can influe...