Standardized microfluidic assessment of red blood cell–mediated microcapillary occlusion: Association with clinical phenotype and hydroxyurea responsiveness in sickle cell disease

• Published in: Microcirculation (New York, N.Y. 1994) Vol. 28; no. 2; pp. e12662 - n/a
• Main Authors: Man, Yuncheng, Kucukal, Erdem, An, Ran, Bode, Allison, Little, Jane A., Gurkan, Umut A.
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.02.2021
• Subjects: Anemia, Sickle Cell - drug therapy; Blood; Erythrocytes; Genotype & phenotype; Hemoglobin; Hemoglobins; Humans; Hydroxyurea - therapeutic use; Microcirculation; Microfluidics; microvascular occlusion; Phenotype; Sickle cell disease; microvascular occlusion; microcirculation; microfluidics; Erythrocytes; sickle cell disease

Objectives We present a standardized in vitro microfluidic assay and Occlusion Index (OI) for the assessment of red blood cell (RBC)–mediated microcapillary occlusion and its clinical associations in sickle cell disease (SCD). Methods Red blood cell mediated microcapillary occlusion represented by O...