Ferguson plots based on absolute mobilities in polyacrylamide gel electrophoresis: dependence of linearity of polymerization conditions and application to the determination of free mobility

• Published in: Electrophoresis Vol. 9; no. 7; p. 293
• Main Authors: Butterman, M, Tietz, D, Orbán, L, Chrambach, A
• Format: Journal Article
• Language: English
• Published: Germany 1988
• Subjects: Automatic Data Processing; Buffers; Electrophoresis, Polyacrylamide Gel - instrumentation; Mathematics; Polymers; Proteins - analysis

In contrast to Ferguson plots based on relative mobilities, Ferguson plots of proteins in polyacrylamide gel electrophoresis based on their absolute mobilities were found to be linear under unusual polymerization conditions which yield relatively wide gel fibers and a low total fiber length per unit...