Site‐specific and endothelial‐mediated dysfunction of the alveolar‐capillary barrier in response to lipopolysaccharides

• Published in: Journal of cellular and molecular medicine Vol. 22; no. 2; pp. 982 - 998
• Main Authors: Janga, Harshavardhan, Cassidy, Liam, Wang, Fanlu, Spengler, Dietmar, Oestern‐Fitschen, Stefanie, Krause, Martin F., Seekamp, Andreas, Tholey, Andreas, Fuchs, Sabine
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01.02.2018; John Wiley and Sons Inc
• Subjects: Adult; alveolar‐capillary barrier of the lung; Alveoli; Angiopoietin; Capillaries - drug effects; Capillaries - physiopathology; Cathepsin S; Caveolin 1 - metabolism; Cell Line; Cell Membrane Permeability - drug effects; Coculture Techniques; Cytokines; Electric Impedance; Endothelial cells; Endothelial Cells - drug effects; Endothelial Cells - metabolism; Endothelial Cells - pathology; Enzyme-linked immunosorbent assay; Epithelial cells; epithelial–endothelial crosstalk; Gene Expression Regulation - drug effects; Humans; Immunomodulation; Inflammation; Inflammation - pathology; Intercellular adhesion molecule 1; Interleukin 6; Lipopolysaccharides; Lipopolysaccharides - toxicity; Liquid chromatography; Liquid chromatography–mass spectrometry‐based proteomics; Lungs; Mass spectrometry; Mass spectroscopy; Monoculture; Original; Permeability; Phosphorylation - drug effects; Proteins; Proteomics; Pulmonary Alveoli - drug effects; Pulmonary Alveoli - physiopathology; Pulmonary Surfactant-Associated Proteins - metabolism; Tight Junction Proteins - metabolism; Vascular cell adhesion molecule 1; Zonula Occludens-1 Protein - metabolism; Liquid chromatography-mass spectrometry-based proteomics; lipopolysaccharides; alveolar-capillary barrier of the lung; epithelial-endothelial crosstalk

Infectious agents such as lipopolysaccharides (LPS) challenge the functional properties of the alveolar‐capillary barrier (ACB) in the lung. In this study, we analyse the site‐specific effects of LPS on the ACB and reveal the effects on the individual cell types and the ACB as a functional unit. Mon...