Direct detection in clinical samples of multiple gene mutations causing resistance of Mycobacterium tuberculosis to isoniazid and rifampicin using fluorogenic probes

• Published in: Journal of antimicrobial chemotherapy Vol. 55; no. 6; pp. 860 - 865
• Main Authors: Espasa, Mateu, González-Martín, Julián, Alcaide, Fernando, Aragón, Lina Marcela, Lonca, Joan, Manterola, Joxe Mari, Salvadó, Margarita, Tudó, Griselda, Orús, Pilar, Coll, Pere
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01.06.2005; Oxford Publishing Limited (England)
• Subjects: Antibiotics. Antiinfectious agents. Antiparasitic agents; Antitubercular Agents - pharmacology; Biological and medical sciences; detection method; drug resistance; Drug Resistance, Bacterial; Fluorescent Dyes; Humans; Isoniazid - pharmacology; Medical sciences; Mutation; Mycobacterium tuberculosis - drug effects; Mycobacterium tuberculosis - genetics; Pharmacology. Drug treatments; Rifampin - pharmacology; Sensitivity and Specificity; Treatment resistance; Multiple; Method; TB; Antibiotic; Rifampicin; Gene; Mycobacterium tuberculosis; Mycobacteriales; detection method; Mycobacteriaceae; Bacteria; Genetics; Actinomycetes; Antituberculous agent; Diagnosis; Mutation; drug resistance; Antibacterial agent; Detection; Isoniazid

Background: This study evaluates a method based on real-time PCR for direct detection in clinical samples of the common mutations responsible for isoniazid and rifampicin resistance of Mycobacterium tuberculosis. Methods: Six pairs of fluorogenic 5′ exonuclease probes (Taqman®), mutated and wild-typ...