Selection of Leptin Surrogates by a General Phenotypic Screening Method for Receptor Agonists

• Published in: Biomolecules (Basel, Switzerland) Vol. 14; no. 4; p. 457
• Main Authors: Wang, Tao, Chen, Xixi, Yang, Guang, Shi, Xiaojie
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.04.2024
• Subjects: Agonists; Agonists (Biochemistry); Antibodies; biological receptor activation-dependent cell survival (BRADS); Cell activation; Cell culture; Cell survival; Cell Survival - drug effects; Cloning; cytokine surrogate; Cytokines; Diabetes; Energy consumption; Flow cytometry; Growth factors; HEK293 Cells; high-throughput screening; High-throughput screening (Biochemical assaying); High-Throughput Screening Assays - methods; Humans; Immune system; Leptin; Leptin - metabolism; Ligands; Methods; Phenotype; phenotypic screening; Phosphatase; Physiological aspects; receptor agonist; Receptor mechanisms; Receptors, Leptin - agonists; Receptors, Leptin - metabolism; China; United States > US; phenotypic screening; receptor agonist; biological receptor activation-dependent cell survival (BRADS); high-throughput screening; cytokine surrogate

There is a high demand for agonist biomolecules such as cytokine surrogates in both biological and medicinal research fields. These are typically sourced through natural ligand engineering or affinity-based screening, followed by individual functional validation. However, efficient screening methods...