Detection of ricin activity and structure by using novel galactose-terminated magnetic bead extraction coupled with mass spectrometric detection

• Published in: Analytical biochemistry Vol. 631; p. 114364
• Main Authors: Hoyt, Kaitlin, Barr, John R., Kalb, Suzanne R.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.10.2021
• Subjects: Animals; Antibodies - chemistry; Biotyper; Food Contamination - analysis; Galactose - chemistry; Galactose affinity; Lactase - chemistry; Limit of Detection; Magnetic Phenomena; MALDI; Mass spectrometry; Microspheres; Milk - chemistry; Plant Extracts - analysis; Powders - analysis; Powders - chemistry; Reproducibility of Results; Ricin; Ricin - analysis; Ricin - chemistry; Ricin - metabolism; Ricinus - chemistry; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - instrumentation; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods; Trypsin - chemistry; Biotyper; Galactose affinity; Ricin; Mass spectrometry; MALDI

Ricin is a toxic protein derived from the castor bean plant (Ricinus communis) and has potential for bioterrorism or criminal use. Therefore, sensitive and rapid analytical methods are needed for its confirmatory detection in environmental samples. Our laboratory previously reported on the developme...