miR-497-5p inhibits gastric cancer cell proliferation and growth through targeting PDK3

• Published in: Bioscience reports Vol. 39; no. 9
• Main Authors: Feng, Lan, Cheng, Kai, Zang, Rongjia, Wang, Qingdong, Wang, Jianjie
• Format: Journal Article
• Language: English
• Published: England Portland Press Ltd 30.09.2019
• Subjects: DNA synthesis; PDK3; apoptosis; gastric cancer; cell cycle; miR-497-5p
• ISSN: 0144-8463; 1573-4935; 1573-4935
• DOI: 10.1042/BSR20190654

MicroRNA plays an important role in gastric cancer (GC) development, while the function of miR-497-5p in this disease remains unknown. In the present study, we demonstrated miR-497-5p as a tumor suppressive microRNA in GC. miR-497-5p was down-regulated in GC tissues and its expression was associated with the disease stage. Inhibition of miR-497-5p promoted GC cell proliferation and growth. By contrast, miR-497-5p ectopic expression suppressed the proliferation and growth of GC cells. In addition, miR-497-5p inhibited DNA synthesis and enhanced apoptosis in GC cells. The cell cycle progression was suppressed by miR-497-5p. Mechanistically, miR-497-5p directly targeted and suppressed the expression of pyruvate dehydrogenase kinase 3 (PDK3), which is highly expressed in GC tissues. Over-expression of PDK3 promoted the proliferation of GC cells. Our study revealed that miR-497-5p inhibited GC cell proliferation and growth via targeting PDK3.