Biochemical characterization of G protein coupling to calcitonin gene–related peptide and adrenomedullin receptors using a native PAGE assay

• Published in: The Journal of biological chemistry Vol. 295; no. 28; pp. 9736 - 9751
• Main Authors: Roehrkasse, Amanda M., Warner, Margaret L., Booe, Jason M., Pioszak, Augen A.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 10.07.2020; American Society for Biochemistry and Molecular Biology
• Subjects: Animals; Calcitonin Gene-Related Peptide - chemistry; Calcitonin Gene-Related Peptide - genetics; Calcitonin Gene-Related Peptide - metabolism; Chlorocebus aethiops; COS Cells; Cyclic AMP - metabolism; G protein; G protein-coupled receptor (GPCR); HEK293 Cells; hormone receptor; hrCNE; Humans; in-gel fluorescence; Methods and Resources; mini-G; molecular pharmacology; Native Polyacrylamide Gel Electrophoresis; peptide hormone; Protein Domains; receptor structure/function; Receptors, Adrenomedullin - chemistry; Receptors, Adrenomedullin - genetics; Receptors, Adrenomedullin - metabolism; Second Messenger Systems; thermostability; in-gel fluorescence; thermostability; molecular pharmacology; G protein-coupled receptor (GPCR); receptor structure/function; peptide hormone; mini-G; hormone receptor; hrCNE; G protein

Calcitonin gene-related peptide (CGRP), adrenomedullin (AM), and adrenomedullin 2/intermedin (AM2/IMD) have overlapping and unique functions in the nervous and circulatory systems including vasodilation, cardioprotection, and pain transmission. Their actions are mediated by the class B calcitonin-li...