Using genetically incorporated unnatural amino acids to control protein functions in mammalian cells

• Published in: Essays in biochemistry Vol. 63; no. 2; p. 237
• Main Authors: Nödling, Alexander R, Spear, Luke A, Williams, Thomas L, Luk, Louis Y P, Tsai, Yu-Hsuan
• Format: Journal Article
• Language: English
• Published: England 03.07.2019
• Subjects: Amino Acids - metabolism; Animals; Genetic Code; Genetic Therapy; Mammals - genetics; Protein Engineering - methods; Proteins - metabolism; protein chemistry; protein engineering; genetic code expansion; unnatural amino acid; chemical biology

Genetic code expansion allows unnatural (non-canonical) amino acid incorporation into proteins of interest by repurposing the cellular translation machinery. The development of this technique has enabled site-specific incorporation of many structurally and chemically diverse amino acids, facilitatin...