Leucine and mTORc1 act independently to regulate 2-deoxyglucose uptake in L6 myotubes

• Published in: Amino acids Vol. 52; no. 3; pp. 477 - 486
• Main Authors: Yin, Qiong, Brameld, John M., Parr, Tim, Murton, Andrew J.
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01.03.2020; Springer Nature B.V
• Subjects: AKT protein; Analytical Chemistry; Biochemical Engineering; Biochemistry; Biomedical and Life Sciences; Cell differentiation; Contrast media; Deoxyglucose; Glucose; Impact resistance; Insulin; Insulin resistance; Intracellular signalling; Leucine; Life Sciences; Muscles; Myotubes; Neurobiology; Original Article; Phosphorylation; Proteomics; Rapamycin; Signaling; TOR protein; Obesity; Insulin resistance; Glucose uptake; Muscle; Leucine; Branched chain amino acids

Chronic mTORc1 hyperactivation via obesity-induced hyperleucinaemia has been implicated in the development of insulin resistance, yet the direct impact of leucine on insulin-stimulated glucose uptake in muscle cells remains unclear. To address this, differentiated L6 myotubes were subjected to vario...