Protein O-fucosyltransferase 2–mediated O-glycosylation of the adhesin MIC2 is dispensable for Toxoplasma gondii tachyzoite infection

• Published in: The Journal of biological chemistry Vol. 294; no. 5; pp. 1541 - 1553
• Main Authors: Khurana, Sachin, Coffey, Michael J., John, Alan, Uboldi, Alessandro D., Huynh, My-Hang, Stewart, Rebecca J., Carruthers, Vern B., Tonkin, Christopher J., Goddard-Borger, Ethan D., Scott, Nichollas E.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.02.2019; American Society for Biochemistry and Molecular Biology
• Subjects: Cells, Cultured; Fibroblasts - cytology; Fibroblasts - metabolism; Fibroblasts - parasitology; fucosyltransferase; Fucosyltransferases - metabolism; Glycobiology and Extracellular Matrices; Glycosylation; Host-Parasite Interactions; Humans; Membrane Proteins - metabolism; Proteome - analysis; proteomics; Protozoan Proteins - metabolism; Toxoplasma - pathogenicity; Toxoplasma gondii; Toxoplasmosis - metabolism; Toxoplasmosis - parasitology; glycosylation; fucosyltransferase; Toxoplasma gondii; proteomics; MS
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.RA118.005357

Toxoplasma gondii is a ubiquitous, obligate intracellular eukaryotic parasite that causes congenital birth defects, disease in immunocompromised individuals, and blindness. Protein glycosylation plays an important role in the infectivity and evasion of immune responses of many eukaryotic parasites and is also of great relevance to vaccine design. Here we demonstrate that micronemal protein 2 (MIC2), a motility-associated adhesin of T. gondii, has highly glycosylated thrombospondin repeat (TSR) domains. Using affinity-purified MIC2 and MS/MS analysis along with enzymatic digestion assays, we observed that at least seven C-linked and three O-linked glycosylation sites exist within MIC2, with >95% occupancy at these O-glycosylation sites. We found that addition of O-glycans to MIC2 is mediated by a protein O-fucosyltransferase 2 homolog (TgPOFUT2) encoded by the TGGT1_273550 gene. Even though POFUT2 homologs are important for stabilizing motility-associated adhesins and for host infection in other apicomplexan parasites, loss of TgPOFUT2 in T. gondii had only a modest impact on MIC2 levels and the wider parasite proteome. Consistent with this, both plaque formation and tachyzoite invasion were broadly similar in the presence or absence of TgPOFUT2. These findings indicate that TgPOFUT2 O-glycosylates MIC2 and that this glycan, in contrast to previous findings in another study, is dispensable in T. gondii tachyzoites and for T. gondii infectivity.