Super-rapid quantitation of the production of HIV-1 harboring a luminescent peptide tag

• Published in: The Journal of biological chemistry Vol. 295; no. 37; pp. 13023 - 13030
• Main Authors: Ozono, Seiya, Zhang, Yanzhao, Tobiume, Minoru, Kishigami, Satoshi, Tokunaga, Kenzo
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 11.09.2020; American Society for Biochemistry and Molecular Biology
• Subjects: Genetic Vectors - genetics; Genetic Vectors - metabolism; HeLa Cells; HIV-1; HIV-1 - genetics; HIV-1 - metabolism; human immunodeficiency virus (HIV); Humans; infection; integrase; Luciferases - genetics; Luciferases - metabolism; luminescent peptide tag; Microbiology; Peptides - genetics; Peptides - metabolism; quantitation; Vif; viral protein; viral replication; virology; HIV-1; viral replication; Vif; infection; human immunodeficiency virus (HIV); virology; luminescent peptide tag; quantitation; viral protein; integrase

In studies of HIV-1, virus production is normally monitored by either a reverse transcriptase assay or a p24 antigen capture ELISA. However, these assays are costly and time-consuming for routine handling of a large number of HIV-1 samples. For example, sample dilution is always required in the ELIS...