Hybrid SWATH/MS and HR-SRM/MS acquisition for phospholipidomics using QUAL/QUANT data processing

• Published in: Analytical and bioanalytical chemistry Vol. 411; no. 22; pp. 5681 - 5690
• Main Authors: Raetz, Michel, Duchoslav, Eva, Bonner, Ron, Hopfgartner, Gérard
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.09.2019; Springer; Springer Nature B.V
• Subjects: Analytical Chemistry; Biochemistry; Blood plasma; Calibration; Characterization and Evaluation of Materials; chemical species; Chemistry; Chemistry and Materials Science; chromatography; Chromatography, Liquid - methods; Combinatorial analysis; Composition; Data processing; Food Science; Humans; information processing; Ion currents; Laboratory Medicine; Lipids; Mass Spectrometry - methods; monitoring; Monitoring/Environmental Analysis; Phospholipids; Phospholipids - blood; Phospholipids - classification; Precursors; prediction; Qualitative analysis; Research Paper; Workflow; Plasma; Data processing; SWATH; Glycerophospholipids; HILIC; QUAL/QUANT
• ISSN: 1618-2642; 1618-2650; 1618-2650
• DOI: 10.1007/s00216-019-01946-4

A hybrid SWATH/MS and HR-SRM/MS acquisition approach using multiple unit mass windows and 100 u precursor selection windows has been developed to interface with a chromatographic lipid class separation. The method allows for the simultaneous monitoring of sum compositions in MS1 and up to 48 lipids...