The specific purification of various carbohydrate-binding hemagglutinins
The specific affinity adsorbent prepared by cross-linking the inhibitory sugar of the hemagglutinin to be purified to insoluble starch with epichlorhydrin was applied to the purification of various carbohydrate-binding hemagglutinins. The hemagglutinin can be isolated in essentially one step, and th...
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| Published in | Biochemical and biophysical research communications Vol. 46; no. 5; pp. 1810 - 1815 |
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| Main Authors | , |
| Format | Journal Article |
| Language | English |
| Published |
United States
Elsevier Inc
10.03.1972
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| Subjects | |
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| Abstract | The specific affinity adsorbent prepared by cross-linking the inhibitory sugar of the hemagglutinin to be purified to insoluble starch with epichlorhydrin was applied to the purification of various carbohydrate-binding hemagglutinins. The hemagglutinin can be isolated in essentially one step, and the adsorbent can be used several times with little changes in its capacity. If the effective inhibitory substance for the hemagglutinin is glycoprotein, the adsorbent prepared by coupling the glycoprotein to activated Sepharose is recommended for the purification of the hemagglutinin. |
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| AbstractList | The specific affinity adsorbent prepared by cross-linking the inhibitory sugar of the hemagglutinin to be purified to insoluble starch with epichlorhydrin was applied to the purification of various carbohydrate-binding hemagglutinins. The hemagglutinin can be isolated in essentially one step, and the adsorbent can be used several times with little changes in its capacity. If the effective inhibitory substance for the hemagglutinin is glycoprotein, the adsorbent prepared by coupling the glycoprotein to activated Sepharose is recommended for the purification of the hemagglutinin. |
| Author | Osawa, Toshiaki Matsumoto, Isamu |
| Author_xml | – sequence: 1 givenname: Isamu surname: Matsumoto fullname: Matsumoto, Isamu – sequence: 2 givenname: Toshiaki surname: Osawa fullname: Osawa, Toshiaki |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/5062716$$D View this record in MEDLINE/PubMed |
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| References | Etzler, Kabat (BIB4) 1970; 9 Kristiansen, Sundberg, Porath (BIB5) 1969; 184 Watkins, Morgan (BIB8) 1962; 7 Khalap, Thompson, Gold (BIB2) 1970; 18 Matsumoto, Osawa (BIB9) 1970; 140 Toyoshima, S., Fukuda, M. and Osawa, T., manuscript in preparation. Borberg, Woodruff, Hirschhorn, Gesner, Miescher, Silber (BIB10) 1966; 154 Hammarström, Kabat (BIB3) 1969; 8 Morgan, Watkins (BIB6) 1953; 34 Boyd (BIB1) 1963; 8 Matsumoto, Osawa (BIB7) 1969; 194 Boyd (10.1016/0006-291X(72)90055-1_BIB1) 1963; 8 Hammarström (10.1016/0006-291X(72)90055-1_BIB3) 1969; 8 Matsumoto (10.1016/0006-291X(72)90055-1_BIB7) 1969; 194 Matsumoto (10.1016/0006-291X(72)90055-1_BIB9) 1970; 140 Watkins (10.1016/0006-291X(72)90055-1_BIB8) 1962; 7 Kristiansen (10.1016/0006-291X(72)90055-1_BIB5) 1969; 184 Khalap (10.1016/0006-291X(72)90055-1_BIB2) 1970; 18 Borberg (10.1016/0006-291X(72)90055-1_BIB10) 1966; 154 10.1016/0006-291X(72)90055-1_BIB11 Etzler (10.1016/0006-291X(72)90055-1_BIB4) 1970; 9 Morgan (10.1016/0006-291X(72)90055-1_BIB6) 1953; 34 |
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| SubjectTerms | Acetates Animals Antibodies - isolation & purification Chemical Phenomena Chemistry Chromatography, Affinity Cyanogen Bromide Eels Ethers, Cyclic Fucose Galactosamine Glycoproteins Lectins - isolation & purification Oligosaccharides plants Polysaccharides Starch Thyroglobulin |
| Title | The specific purification of various carbohydrate-binding hemagglutinins |
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