m6A RNA modifications are measured at single-base resolution across the mammalian transcriptome

• Published in: Nature biotechnology Vol. 40; no. 8; pp. 1210 - 1219
• Main Authors: Hu, Lulu, Liu, Shun, Peng, Yong, Ge, Ruiqi, Su, Rui, Senevirathne, Chamara, Harada, Bryan T., Dai, Qing, Wei, Jiangbo, Zhang, Lisheng, Hao, Ziyang, Luo, Liangzhi, Wang, Huanyu, Wang, Yuru, Luo, Minkui, Chen, Mengjie, Chen, Jianjun, He, Chuan
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.08.2022; Nature Publishing Group
• Subjects: 631/45/500; 631/61/514/1949; Agriculture; Antibodies; Bioinformatics; Biological activity; Biology; Biomedical and Life Sciences; Biomedical Engineering/Biotechnology; Biomedicine; Biotechnology; Calibration; Cancer; Cell differentiation; Cell lines; Cells (biology); Differentiation (biology); Enzymes; Epigenetics; Gene expression; Hematopoietic stem cells; HIV; Human immunodeficiency virus; Labeling; Laboratories; Life Sciences; Mammals; Methylation; Mutation; N6-methyladenosine; Nucleotides; Progenitor cells; Quantitative analysis; RNA modification; rRNA; Stoichiometry; Transcription factors; Transcriptomes

Functional studies of the RNA N 6 -methyladenosine (m 6 A) modification have been limited by an inability to map individual m 6 A-modified sites in whole transcriptomes. To enable such studies, here, we introduce m 6 A-selective allyl chemical labeling and sequencing (m 6 A-SAC-seq), a method for qu...