The autophagy protein Ambra1 regulates gene expression by supporting novel transcriptional complexes

• Published in: The Journal of biological chemistry Vol. 295; no. 34; pp. 12045 - 12057
• Main Authors: Schoenherr, Christina, Byron, Adam, Griffith, Billie, Loftus, Alexander, Wills, Jimi C., Munro, Alison F., von Kriegsheim, Alex, Frame, Margaret C.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 21.08.2020; American Society for Biochemistry and Molecular Biology
• Subjects: A Kinase Anchor Proteins - genetics; A Kinase Anchor Proteins - metabolism; Activating Transcription Factor 2 - genetics; Activating Transcription Factor 2 - metabolism; Active Transport, Cell Nucleus - genetics; Adaptor Proteins, Signal Transducing - genetics; Adaptor Proteins, Signal Transducing - metabolism; Akap8; Ambra1; Angiopoietin-1 - biosynthesis; Angiopoietin-1 - genetics; Atf2; autophagy; Cdk9; Cell Biology; Cell Line; chromatin; Chromatin - genetics; Chromatin - metabolism; DNA Helicases - genetics; DNA Helicases - metabolism; DNA transcription; Focal Adhesion Kinase 1 - genetics; Focal Adhesion Kinase 1 - metabolism; Gene Expression Regulation; Humans; Integrin alpha Chains - biosynthesis; Integrin alpha Chains - genetics; Integrin beta Chains - biosynthesis; Integrin beta Chains - genetics; Multiprotein Complexes - genetics; Multiprotein Complexes - metabolism; Nuclear Proteins - genetics; Nuclear Proteins - metabolism; nucleus; Signal Transduction; trafficking; transcription; Transcription Factors - genetics; Transcription Factors - metabolism; Transforming Growth Factor beta2 - biosynthesis; Transforming Growth Factor beta2 - genetics; Transforming Growth Factor beta3 - biosynthesis; Transforming Growth Factor beta3 - genetics; Atf2; Cdk9; autophagy; transcription; Ambra1; nucleus; Akap8; chromatin; trafficking; DNA transcription

Ambra1 is considered an autophagy and trafficking protein with roles in neurogenesis and cancer cell invasion. Here, we report that Ambra1 also localizes to the nucleus of cancer cells, where it has a novel nuclear scaffolding function that controls gene expression. Using biochemical fractionation a...