Lacritin proteoforms prevent tear film collapse and maintain epithelial homeostasis

• Published in: The Journal of biological chemistry Vol. 296; p. 100070
• Main Authors: Georgiev, Georgi A., Gh, Mohammad Sharifian, Romano, Jeff, Dias Teixeira, Karina L., Struble, Craig, Ryan, Denise S., Sia, Rose K., Kitt, Jay P., Harris, Joel M., Hsu, Ku-Lung, Libby, Adam, Odrich, Marc G., Suárez, Tatiana, McKown, Robert L., Laurie, Gordon W.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.01.2021; American Society for Biochemistry and Molecular Biology
• Subjects: elastic modulus; epithelium; eye; lacritin; OAHFA; protein–lipid interaction; proteoform; proteolysis; Raman spectroscopy; tears; FOXC2; tears; OAHFA; protein–lipid interaction; NGLY1; AAAS; Cyp4f39; lacritin; EMC3; TRAPPC11; proteolysis; TP63; proteoform; SFTPB; ABCA3; SFTPC; HCE-T; EIM; Raman spectroscopy; ER; eye; CLDN10; elastic modulus; FWHM; epithelium; TGFBI; FGFR3; FGFR2; FGF10; fatty acid; autoimmune disease; homeostasis; protein turnover; protein-lipid interaction; mass spectrometry (MS)

Lipids in complex, protein-enriched films at air/liquid interfaces reduce surface tension. In the absence of this benefit, the light refracting and immunoprotective tear film on eyes would collapse. Premature collapse, coupled with chronic inflammation compromising visual acuity, is a hallmark of dr...