Chemoenzymatic Synthesis of Prenylated Indole Derivatives by Using a 4-Dimethylallyltryptophan Synthase from Aspergillus fumigatus

• Published in: Chembiochem : a European journal of chemical biology Vol. 8; no. 11; pp. 1298 - 1307
• Main Authors: Steffan, Nicola, Unsöld, Inge A., Li, Shu-Ming
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 23.07.2007; WILEY‐VCH Verlag
• Subjects: alkaloids; Alkyl and Aryl Transferases - genetics; Alkyl and Aryl Transferases - isolation & purification; Alkyl and Aryl Transferases - metabolism; Aspergillus fumigatus; Aspergillus fumigatus - enzymology; chemoenzymatic synthesis; Chromatography, High Pressure Liquid; Cloning, Molecular; Ergot Alkaloids - biosynthesis; Gene Expression; Indoles - chemical synthesis; Indoles - chemistry; Indoles - metabolism; Kinetics; Magnetic Resonance Spectroscopy; Mass Spectrometry; Molecular Structure; Neoprene - chemistry; Recombinant Proteins - genetics; Recombinant Proteins - isolation & purification; Recombinant Proteins - metabolism; Substrate Specificity; synthases; transferases

A 4‐dimethylallyltryptophan synthase, FgaPT2, has been identified in the genome of Aspergillus fumigatus. In a previous study, FgaPT2 was overexpressed in Saccharomyces cerevisiae and characterized biochemically. A higher protein yield (up to 100‐fold higher than that for S. cerevisiae) has now been...