Topological Distribution of Two Connexin32 Antigenic Sites in Intact and Split Rodent Hepatocyte Gap Junctions

• Published in: The Journal of cell biology Vol. 107; no. 5; pp. 1817 - 1824
• Main Authors: Goodenough, Daniel A., Paul, David L., Jesaitis, Lynne
• Format: Journal Article
• Language: English
• Published: New York, NY Rockefeller University Press 01.11.1988; The Rockefeller University Press
• Subjects: Amino Acid Sequence; Amino acids; Animals; Antibodies, Monoclonal; Antigens; Biological and medical sciences; Blotting, Western; Cell Fractionation - methods; Cell interactions, adhesion; Cells; connexin32; Connexins; Epitopes; Fundamental and applied biological sciences. Psychology; Gap junctions; Hepatocytes; Immunohistochemistry; Intercellular Junctions - immunology; Intercellular Junctions - ultrastructure; Liver; Liver - cytology; Membrane Proteins - immunology; Mice; Molecular and cellular biology; Molecular Sequence Data; Molecules; Oligopeptides - chemical synthesis; P branes; Protein Conformation; Rats; Topology; Antigenic determinant; Rat; Rodentia; Cell junction; Cell cell interaction; Monoclonal antibody; Electron microscopy; Gap junction; Immunological method; Vertebrata; Mammalia; Molecular orientation; Hepatocyte; Mouse; Plasma membrane

The membrane topology of connexin32, a principal polypeptide of gap junctions in diverse cell types, has been studied in rat and mouse hepatocyte gap junctions using site-specific antisera raised against synthetic oligopeptides corresponding to amino acid sequences deduced from cDNA clones. Based on...