Reduction of protein concentration range difference followed by multicolumn fractionation prior to 2-DE and LC-MS/MS profiling of serum proteins - Selvaraju, Subhashini; El Rassi, Ziad (Electrophoresis, 2011)
Keywords: 2-DE; affinity chromatography; Beads; benzene; blood proteins; Blood Proteins - analysis; Blood Proteins - metabolism; blood serum; Chromatography, Affinity - methods; Chromatography, Reverse-Phase; Copper; Distillation; electrophoresis; Electrophoresis, Gel, Two-Dimensional - methods; Equalization; Equalizers; Fractionation; Humans; Immobilized metal ion affinity chromatography; metal ions; Nickel; Peptide Fragments - analysis; Peptide Fragments - classification; Peptide Fragments - metabolism; Peptide Library; polystyrenes; Proteins; Proteomics; Proteomics - methods; ProteoMiner; Reduction; Serums; Tandem Mass Spectrometry - methods; Trypsin - metabolism; Zinc