Analysis of human leukaemias and lymphomas using extensive immunophenotypes from an antibody microarray

• Published in: British journal of haematology Vol. 135; no. 2; pp. 184 - 197
• Main Authors: Belov, Larissa, Mulligan, Stephen P., Barber, Nicole, Woolfson, Adrian, Scott, Mike, Stoner, Kerryn, Chrisp, Jeremy S., Sewell, William A., Bradstock, Kenneth F., Bendall, Linda, Pascovici, Dana S., Thomas, Mervyn, Erber, Wendy, Huang, Pauline, Sartor, Mary, Young, Graham A.R., Wiley, James S., Juneja, Surender, Wierda, William G., Green, Anthony R., Keating, Michael J., Christopherson, Richard I.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.10.2006; Blackwell
• Subjects: Acute Disease; Antigens, CD - blood; Antigens, Neoplasm - blood; array; Biological and medical sciences; Bone Marrow - immunology; CD antigens; Diagnosis, Differential; expression profile; Flow Cytometry; Hematologic and hematopoietic diseases; Humans; immunophenotype; Immunophenotyping - methods; leukaemia; Leukemia - classification; Leukemia - diagnosis; Leukemia - immunology; Leukemia, Lymphocytic, Chronic, B-Cell - diagnosis; Leukemia, Lymphocytic, Chronic, B-Cell - immunology; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis; Lymphoma - classification; Lymphoma - diagnosis; Lymphoma - immunology; Lymphoma, B-Cell - classification; Lymphoma, B-Cell - diagnosis; Lymphoma, B-Cell - immunology; Medical sciences; Protein Array Analysis - methods; Human; Hematology; Immunophenotype; Antibody; Leukemia; DNA chip; Malignant hemopathy; Lymphoid neoplasm; Lymphoma; Antigen; CD antigens; array; Lymphoproliferative syndrome; leukaemia; expression profile

Summary A novel antibody microarray has been developed that provides an extensive immunophenotype of leukaemia cells. The assay is a solid phase cell‐capture technique in which 82 antigens are studied simultaneously. This paper presents the analysis of 733 patients with a variety of leukaemias and l...