Massively-multiplexed epitope mapping techniques for viral antigen discovery

Following viral infection, viral antigens bind specifically to receptors on the surface of lymphocytes thereby activating adaptive immunity in the host. An epitope, the smallest structural and functional unit of an antigen, binds specifically to an antibody or antigen receptor, to serve as key sites...

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Published inFrontiers in immunology Vol. 14; p. 1192385
Main Authors Hu, Diya, Irving, Aaron T.
Format Journal Article
LanguageEnglish
Published Frontiers Media S.A 25.09.2023
Subjects
B cell
epitope
epitope mapping
Immunology
phage-display
T cell
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Abstract Following viral infection, viral antigens bind specifically to receptors on the surface of lymphocytes thereby activating adaptive immunity in the host. An epitope, the smallest structural and functional unit of an antigen, binds specifically to an antibody or antigen receptor, to serve as key sites for the activation of adaptive immunity. The complexity and diverse range of epitopes are essential to study and map for the diagnosis of disease, the design of vaccines and for immunotherapy. Mapping the location of these specific epitopes has become a hot topic in immunology and immune therapy. Recently, epitope mapping techniques have evolved to become multiplexed, with the advent of high-throughput sequencing and techniques such as bacteriophage-display libraries and deep mutational scanning. Here, we briefly introduce the principles, advantages, and disadvantages of the latest epitope mapping techniques with examples for viral antigen discovery.
AbstractList Following viral infection, viral antigens bind specifically to receptors on the surface of lymphocytes thereby activating adaptive immunity in the host. An epitope, the smallest structural and functional unit of an antigen, binds specifically to an antibody or antigen receptor, to serve as key sites for the activation of adaptive immunity. The complexity and diverse range of epitopes are essential to study and map for the diagnosis of disease, the design of vaccines and for immunotherapy. Mapping the location of these specific epitopes has become a hot topic in immunology and immune therapy. Recently, epitope mapping techniques have evolved to become multiplexed, with the advent of high-throughput sequencing and techniques such as bacteriophage-display libraries and deep mutational scanning. Here, we briefly introduce the principles, advantages, and disadvantages of the latest epitope mapping techniques with examples for viral antigen discovery.
Author Hu, Diya
Irving, Aaron T.
AuthorAffiliation 2 Department of Clinical Laboratory Studies, Second Affiliated Hospital, Zhejiang University School of Medicine , Hangzhou , China
5 College of Medicine & Veterinary Medicine, The University of Edinburgh , Edinburgh , United Kingdom
3 Centre for Infection, Immunity & Cancer, Zhejiang University-University of Edinburgh Institute, Zhejiang University School of Medicine, Zhejiang University , Haining , China
4 Biomedical and Health Translational Research Centre of Zhejiang Province (BIMET) , Haining , China
1 Zhejiang University-University of Edinburgh Institute, Zhejiang University School of Medicine, Zhejiang University , Haining , China
AuthorAffiliation_xml – name: 1 Zhejiang University-University of Edinburgh Institute, Zhejiang University School of Medicine, Zhejiang University , Haining , China
– name: 4 Biomedical and Health Translational Research Centre of Zhejiang Province (BIMET) , Haining , China
– name: 5 College of Medicine & Veterinary Medicine, The University of Edinburgh , Edinburgh , United Kingdom
– name: 3 Centre for Infection, Immunity & Cancer, Zhejiang University-University of Edinburgh Institute, Zhejiang University School of Medicine, Zhejiang University , Haining , China
– name: 2 Department of Clinical Laboratory Studies, Second Affiliated Hospital, Zhejiang University School of Medicine , Hangzhou , China
Author_xml – sequence: 1
  givenname: Diya
  surname: Hu
  fullname: Hu, Diya
– sequence: 2
  givenname: Aaron T.
  surname: Irving
  fullname: Irving, Aaron T.
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CitedBy_id crossref_primary_10_1016_j_tibtech_2024_04_003
crossref_primary_10_1186_s12985_024_02424_9
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Edited by: Elizabeth Secord, Wayne State University, United States
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Snippet Following viral infection, viral antigens bind specifically to receptors on the surface of lymphocytes thereby activating adaptive immunity in the host. An...
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SubjectTerms B cell
epitope
epitope mapping
Immunology
phage-display
T cell
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Title Massively-multiplexed epitope mapping techniques for viral antigen discovery
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