Improved vectors for stable expression of foreign genes in mammalian cells by use of the untranslated leader sequence from EMC virus

• Published in: Nucleic acids research Vol. 19; no. 16; pp. 4485 - 4490
• Main Authors: Kaufman, R J, Davies, M V, Wasley, L C, Michnick, D
• Format: Journal Article
• Language: English
• Published: England 25.08.1991
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Cell Line; Cricetinae; Cricetulus; encephalomyocarditis virus; Encephalomyocarditis virus - genetics; Gene Expression; Genetic Vectors - genetics; Haplorhini; Kanamycin Kinase; Mice; Molecular Sequence Data; Open Reading Frames - genetics; Phosphotransferases - genetics; Protein Biosynthesis; Rats; Tetrahydrofolate Dehydrogenase - genetics; Transfection

Dicistronic mRNA expression vectors efficiently translate a 5' open reading frame (ORF) and contain a selectable marker within the 3' end which is inefficiently translated. In these vectors, the efficiency of translation of the selectable 3' ORF is reduced approximately 100-fold and i...