Developing a Silk Fibroin Composite Film to Scavenge and Probe H2O2 Associated with UV-Excitable Blue Fluorescence
A silk fibroin composite film that can simultaneously scavenge and probe H2O2 in situ was developed for possibly examining local concentrations of H2O2 for biomedical applications. A multi-functional composite film (GDES) that consists of graphene oxide (G), a photothermally responsive element that...
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| Published in | Sensors (Basel, Switzerland) Vol. 20; no. 2; p. 366 |
|---|---|
| Main Authors | , , , , , , |
| Format | Journal Article |
| Language | English |
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08.01.2020
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| Abstract | A silk fibroin composite film that can simultaneously scavenge and probe H2O2 in situ was developed for possibly examining local concentrations of H2O2 for biomedical applications. A multi-functional composite film (GDES) that consists of graphene oxide (G), a photothermally responsive element that was blended with polydopamine (PDA, D)/horseradish peroxidase (HRP, E) (or DE complex), and then GDE microaggregates were coated with silk fibroin (SF, S), a tyrosine-containing protein. At 37 °C, the H2O2-scavenging ability of a GDES film in solution at approximately 7.5 × 10−3 μmol H2O2/mg film was the highest compared with those of S and GS films. The intensities of UV-excitable blue fluorescence of a GDES film linearly increased with increasing H2O2 concentrations from 4.0 μM to 80 μM at 37 °C. Interestingly, after a GDES film scavenged H2O2, the UV-excitable blue fluorescent film could be qualitatively monitored by eye, making the film an eye-probe H2O2 sensor. A GDES film enabled to heat H2O2-containing samples to 37 °C or higher by the absorption of near-IR irradiation at 808 nm. The good biocompatibility of a GDES film was examined according to the requirements of ISO-10993-5. Accordingly, a GDES film was developed herein to scavenge and eye-probe H2O2 in situ and so it has potential for biomedical applications. |
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| AbstractList | A silk fibroin composite film that can simultaneously scavenge and probe H2O2 in situ was developed for possibly examining local concentrations of H2O2 for biomedical applications. A multi-functional composite film (GDES) that consists of graphene oxide (G), a photothermally responsive element that was blended with polydopamine (PDA, D)/horseradish peroxidase (HRP, E) (or DE complex), and then GDE microaggregates were coated with silk fibroin (SF, S), a tyrosine-containing protein. At 37 °C, the H2O2-scavenging ability of a GDES film in solution at approximately 7.5 × 10−3 μmol H2O2/mg film was the highest compared with those of S and GS films. The intensities of UV-excitable blue fluorescence of a GDES film linearly increased with increasing H2O2 concentrations from 4.0 μM to 80 μM at 37 °C. Interestingly, after a GDES film scavenged H2O2, the UV-excitable blue fluorescent film could be qualitatively monitored by eye, making the film an eye-probe H2O2 sensor. A GDES film enabled to heat H2O2-containing samples to 37 °C or higher by the absorption of near-IR irradiation at 808 nm. The good biocompatibility of a GDES film was examined according to the requirements of ISO-10993-5. Accordingly, a GDES film was developed herein to scavenge and eye-probe H2O2 in situ and so it has potential for biomedical applications. A silk fibroin composite film that can simultaneously scavenge and probe H 2 O 2 in situ was developed for possibly examining local concentrations of H 2 O 2 for biomedical applications. A multi-functional composite film (GDES) that consists of graphene oxide (G), a photothermally responsive element that was blended with polydopamine (PDA, D)/horseradish peroxidase (HRP, E) (or DE complex), and then GDE microaggregates were coated with silk fibroin (SF, S), a tyrosine-containing protein. At 37 °C, the H 2 O 2 -scavenging ability of a GDES film in solution at approximately 7.5 × 10 −3 μmol H 2 O 2 /mg film was the highest compared with those of S and GS films. The intensities of UV-excitable blue fluorescence of a GDES film linearly increased with increasing H 2 O 2 concentrations from 4.0 μM to 80 μM at 37 °C. Interestingly, after a GDES film scavenged H 2 O 2 , the UV-excitable blue fluorescent film could be qualitatively monitored by eye, making the film an eye-probe H 2 O 2 sensor. A GDES film enabled to heat H 2 O 2 -containing samples to 37 °C or higher by the absorption of near-IR irradiation at 808 nm. The good biocompatibility of a GDES film was examined according to the requirements of ISO-10993-5. Accordingly, a GDES film was developed herein to scavenge and eye-probe H 2 O 2 in situ and so it has potential for biomedical applications. A silk fibroin composite film that can simultaneously scavenge and probe H2O2 in situ was developed for possibly examining local concentrations of H2O2 for biomedical applications. A multi-functional composite film (GDES) that consists of graphene oxide (G), a photothermally responsive element that was blended with polydopamine (PDA, D)/horseradish peroxidase (HRP, E) (or DE complex), and then GDE microaggregates were coated with silk fibroin (SF, S), a tyrosine-containing protein. At 37 °C, the H2O2-scavenging ability of a GDES film in solution at approximately 7.5 × 10-3 μmol H2O2/mg film was the highest compared with those of S and GS films. The intensities of UV-excitable blue fluorescence of a GDES film linearly increased with increasing H2O2 concentrations from 4.0 μM to 80 μM at 37 °C. Interestingly, after a GDES film scavenged H2O2, the UV-excitable blue fluorescent film could be qualitatively monitored by eye, making the film an eye-probe H2O2 sensor. A GDES film enabled to heat H2O2-containing samples to 37 °C or higher by the absorption of near-IR irradiation at 808 nm. The good biocompatibility of a GDES film was examined according to the requirements of ISO-10993-5. Accordingly, a GDES film was developed herein to scavenge and eye-probe H2O2 in situ and so it has potential for biomedical applications.A silk fibroin composite film that can simultaneously scavenge and probe H2O2 in situ was developed for possibly examining local concentrations of H2O2 for biomedical applications. A multi-functional composite film (GDES) that consists of graphene oxide (G), a photothermally responsive element that was blended with polydopamine (PDA, D)/horseradish peroxidase (HRP, E) (or DE complex), and then GDE microaggregates were coated with silk fibroin (SF, S), a tyrosine-containing protein. At 37 °C, the H2O2-scavenging ability of a GDES film in solution at approximately 7.5 × 10-3 μmol H2O2/mg film was the highest compared with those of S and GS films. The intensities of UV-excitable blue fluorescence of a GDES film linearly increased with increasing H2O2 concentrations from 4.0 μM to 80 μM at 37 °C. Interestingly, after a GDES film scavenged H2O2, the UV-excitable blue fluorescent film could be qualitatively monitored by eye, making the film an eye-probe H2O2 sensor. A GDES film enabled to heat H2O2-containing samples to 37 °C or higher by the absorption of near-IR irradiation at 808 nm. The good biocompatibility of a GDES film was examined according to the requirements of ISO-10993-5. Accordingly, a GDES film was developed herein to scavenge and eye-probe H2O2 in situ and so it has potential for biomedical applications. |
| Author | Chen, Weng-Pin Jan, Yun-Jen Chung, Tze-Wen Chang, Chun-Ning Liao, Chiu-Hsun Chang, Chun-Yi Chen, Li-Ting |
| AuthorAffiliation | 5 Additive Manufacturing Center for Mass Customization Production, National Taipei University of Technology, Taipei 10608, Taiwan 1 Department of Biomedical Engineering, National Yang-Ming University, No.155, Sec.2, Linong Street, Taipei 11221, Taiwan; 30304016@gm.ym.edu.tw (C.-Y.C.); ase830217@gmail.com (C.-N.C.); liting0515@gmail.com (L.-T.C.) 4 Department of Mechanical Engineering, National Taipei University of Technology, Taipei 10608, Taiwan 2 The Center for Advanced Pharmaceutics and Drug Delivery Research, National Yang-Ming University, Taipei 11221, Taiwan 3 Miaoli District Agricultural Research and Extension Station, Council of Agriculture, Executive Yuan, Miaoli County 36346, Taiwan; jsliaw@mdais.gov.tw (C.-H.L.); or |
| AuthorAffiliation_xml | – name: 5 Additive Manufacturing Center for Mass Customization Production, National Taipei University of Technology, Taipei 10608, Taiwan – name: 3 Miaoli District Agricultural Research and Extension Station, Council of Agriculture, Executive Yuan, Miaoli County 36346, Taiwan; jsliaw@mdais.gov.tw (C.-H.L.); or – name: 1 Department of Biomedical Engineering, National Yang-Ming University, No.155, Sec.2, Linong Street, Taipei 11221, Taiwan; 30304016@gm.ym.edu.tw (C.-Y.C.); ase830217@gmail.com (C.-N.C.); liting0515@gmail.com (L.-T.C.) – name: 4 Department of Mechanical Engineering, National Taipei University of Technology, Taipei 10608, Taiwan – name: 2 The Center for Advanced Pharmaceutics and Drug Delivery Research, National Yang-Ming University, Taipei 11221, Taiwan |
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| SubjectTerms | Biosensors blue fluorescence Cell growth Dopamine Enzymes Fourier transforms Graphene graphene oxide Graphite h2o2 probe h2o2 scavenge Hydrogels Laboratories Nanoparticles Polymerization Scanning electron microscopy silk fibroin Software |
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| Title | Developing a Silk Fibroin Composite Film to Scavenge and Probe H2O2 Associated with UV-Excitable Blue Fluorescence |
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