SARS-CoV-2 molecular testing and whole genome sequencing following RNA recovery from used BinaxNOW COVID-19 antigen self tests
•SARS-CoV-2 RNA from used Binax test swabs collected and stored at home can be detected by rRT-PCR and used for genotyping and full viral genome sequencing.•Used Binax test swabs provide an important opportunity to improve population-level SARS-CoV-2 genomic surveillance, as an increasing amount of...
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| Published in | Journal of clinical virology Vol. 162; p. 105426 |
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| Main Authors | , , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Netherlands
Elsevier B.V
01.05.2023
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| Abstract | •SARS-CoV-2 RNA from used Binax test swabs collected and stored at home can be detected by rRT-PCR and used for genotyping and full viral genome sequencing.•Used Binax test swabs provide an important opportunity to improve population-level SARS-CoV-2 genomic surveillance, as an increasing amount of testing is performed at home.•SARS-CoV-2 sequences generated from used Binax test swabs can be used to evaluate transmission clusters, identify travel-associated infections, and monitor within-patient evolution.
Widespread use of over-the-counter rapid diagnostic tests for SARS-CoV-2 has led to a decrease in availability of clinical samples for viral genomic surveillance. As an alternative sample source, we evaluated RNA isolated from BinaxNOW swabs stored at ambient temperature for SARS-CoV-2 rRT-PCR and full viral genome sequencing. 81 of 103 samples (78.6%) yielded detectable RNA, and 46 of 57 samples (80.7 %) yielded complete genome sequences. Our results illustrate that SARS-CoV-2 RNA extracted from used Binax test swabs provides an important opportunity for improving SARS-CoV-2 genomic surveillance, evaluating transmission clusters, and monitoring within-patient evolution. |
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| AbstractList | Widespread use of over-the-counter rapid diagnostic tests for SARS-CoV-2 has led to a decrease in availability of clinical samples for viral genomic surveillance. As an alternative sample source, we evaluated RNA isolated from BinaxNOW swabs stored at ambient temperature for SARS-CoV-2 rRT-PCR and full viral genome sequencing. 81 of 103 samples (78.6%) yielded detectable RNA, and 46 of 57 samples (80.7 %) yielded complete genome sequences. Our results illustrate that SARS-CoV-2 RNA extracted from used Binax test swabs provides an important opportunity for improving SARS-CoV-2 genomic surveillance, evaluating transmission clusters, and monitoring within-patient evolution.Widespread use of over-the-counter rapid diagnostic tests for SARS-CoV-2 has led to a decrease in availability of clinical samples for viral genomic surveillance. As an alternative sample source, we evaluated RNA isolated from BinaxNOW swabs stored at ambient temperature for SARS-CoV-2 rRT-PCR and full viral genome sequencing. 81 of 103 samples (78.6%) yielded detectable RNA, and 46 of 57 samples (80.7 %) yielded complete genome sequences. Our results illustrate that SARS-CoV-2 RNA extracted from used Binax test swabs provides an important opportunity for improving SARS-CoV-2 genomic surveillance, evaluating transmission clusters, and monitoring within-patient evolution. •SARS-CoV-2 RNA from used Binax test swabs collected and stored at home can be detected by rRT-PCR and used for genotyping and full viral genome sequencing.•Used Binax test swabs provide an important opportunity to improve population-level SARS-CoV-2 genomic surveillance, as an increasing amount of testing is performed at home.•SARS-CoV-2 sequences generated from used Binax test swabs can be used to evaluate transmission clusters, identify travel-associated infections, and monitor within-patient evolution. Widespread use of over-the-counter rapid diagnostic tests for SARS-CoV-2 has led to a decrease in availability of clinical samples for viral genomic surveillance. As an alternative sample source, we evaluated RNA isolated from BinaxNOW swabs stored at ambient temperature for SARS-CoV-2 rRT-PCR and full viral genome sequencing. 81 of 103 samples (78.6%) yielded detectable RNA, and 46 of 57 samples (80.7 %) yielded complete genome sequences. Our results illustrate that SARS-CoV-2 RNA extracted from used Binax test swabs provides an important opportunity for improving SARS-CoV-2 genomic surveillance, evaluating transmission clusters, and monitoring within-patient evolution. Widespread use of over-the-counter rapid diagnostic tests for SARS-CoV-2 has led to a decrease in availability of clinical samples for viral genomic surveillance. As an alternative sample source, we evaluated RNA isolated from BinaxNOW swabs stored at ambient temperature for SARS-CoV-2 rRT-PCR and full viral genome sequencing. 81 of 103 samples (78.6%) yielded detectable RNA, and 46 of 57 samples (80.7 %) yielded complete genome sequences. Our results illustrate that SARS-CoV-2 RNA extracted from used Binax test swabs provides an important opportunity for improving SARS-CoV-2 genomic surveillance, evaluating transmission clusters, and monitoring within-patient evolution. |
| ArticleNumber | 105426 |
| Author | Wang, Ethan Waggoner, Jesse J. Carmola, Ludy Registre Nguyen, Phuong-Vi Piantadosi, Anne Lam, Wilbur A. Rao, Anuradha Sullivan, Julie A. Martin, Greg S. Greenleaf, Morgan Bassit, Leda |
| Author_xml | – sequence: 1 givenname: Phuong-Vi surname: Nguyen fullname: Nguyen, Phuong-Vi organization: Emory University Department of Medicine, Atlanta, GA, USA – sequence: 2 givenname: Ludy Registre surname: Carmola fullname: Carmola, Ludy Registre organization: Emory University Department of Pathology and Laboratory Medicine, Atlanta, GA, USA – sequence: 3 givenname: Ethan surname: Wang fullname: Wang, Ethan organization: Atlanta Center for Microsystems-Engineered Point-of-Care Technologies, Atlanta, GA, USA – sequence: 4 givenname: Leda surname: Bassit fullname: Bassit, Leda organization: Emory University Department of Pathology and Laboratory Medicine, Atlanta, GA, USA – sequence: 5 givenname: Anuradha surname: Rao fullname: Rao, Anuradha organization: Atlanta Center for Microsystems-Engineered Point-of-Care Technologies, Atlanta, GA, USA – sequence: 6 givenname: Morgan surname: Greenleaf fullname: Greenleaf, Morgan organization: Atlanta Center for Microsystems-Engineered Point-of-Care Technologies, Atlanta, GA, USA – sequence: 7 givenname: Julie A. surname: Sullivan fullname: Sullivan, Julie A. organization: Atlanta Center for Microsystems-Engineered Point-of-Care Technologies, Atlanta, GA, USA – sequence: 8 givenname: Greg S. surname: Martin fullname: Martin, Greg S. organization: Emory University Department of Medicine, Atlanta, GA, USA – sequence: 9 givenname: Wilbur A. surname: Lam fullname: Lam, Wilbur A. organization: Atlanta Center for Microsystems-Engineered Point-of-Care Technologies, Atlanta, GA, USA – sequence: 10 givenname: Jesse J. orcidid: 0000-0002-0244-5957 surname: Waggoner fullname: Waggoner, Jesse J. email: jjwaggo@emory.edu organization: Emory University Department of Medicine, Atlanta, GA, USA – sequence: 11 givenname: Anne orcidid: 0000-0002-5942-1534 surname: Piantadosi fullname: Piantadosi, Anne organization: Emory University Department of Medicine, Atlanta, GA, USA |
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| Snippet | •SARS-CoV-2 RNA from used Binax test swabs collected and stored at home can be detected by rRT-PCR and used for genotyping and full viral genome... Widespread use of over-the-counter rapid diagnostic tests for SARS-CoV-2 has led to a decrease in availability of clinical samples for viral genomic... |
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| SubjectTerms | COVID-19 COVID-19 - diagnosis Humans Molecular Diagnostic Techniques Rapid test RNA, Viral - analysis RNA, Viral - genetics RT-PCR SARS-CoV-2 SARS-CoV-2 - genetics Sequencing Whole Genome Sequencing - methods |
| Title | SARS-CoV-2 molecular testing and whole genome sequencing following RNA recovery from used BinaxNOW COVID-19 antigen self tests |
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