The effect of corticosteroids or nitrogen mustard on aqueous humor chemotactic activity induced by intravitreal endotoxin

• Published in: Clinical immunology and immunopathology Vol. 39; no. 3; pp. 414 - 420
• Main Authors: Rosenbaum, James T., Raymond, Wilfred, Seymour, Brian W.P., Wolfrom, James A.K., Enkel, Holly, Howes, Edward L.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.06.1986; New York, NY Academic Press; Boston
• Subjects: Animals; Applied sciences; Aqueous Humor - physiopathology; Biological and medical sciences; Chemotactic Factors - physiology; Chemotaxis, Leukocyte; Endotoxins; Exact sciences and technology; Eye; Inflammation - physiopathology; Male; Mechlorethamine - pharmacology; Medical sciences; Methylprednisolone - pharmacology; Monocytes - physiology; Other techniques and industries; Pharmacology. Drug treatments; Rabbits; Uveitis - physiopathology; Corticosteroid; Monocyte; Intravitreous administration; Rabbit; Aqueous humor; Lagomorpha; Methylprednisolone; Chemotaxis; Eye; Vertebrata; Mammalia; Leucocyte function; Animal
• DOI: 10.1016/0090-1229(86)90169-8
• ISSN: 0090-1229

In rabbits intravitreal injection of endotoxin induces potent chemotactic activity for monocytes in aqueous humor. We have assessed the effect of methylprednisolone (30 mg/kg intramuscularly) or nitrogen mustard (1.75 mg/kg intravenously) on the generation of this chemotactic activity. Histologic changes, anterior chamber protein extravasation, and aqueous humor cellular infiltration were reduced by corticosteroids to a variable degree. However, even in animals with a marked reduction in protein extravasation or histologic change, chemotactic activity was substantially preserved. Similarly, nitrogen mustard induced a leukopenia without affecting the ability of endotoxin to generate chemotactic activity in aqueous humor. In contrast, corticosteroids reduced both protein extravasation and the generation of chemotactic activity induced by intravenously injected endotoxin. The results suggest that the chemotactic activity induced in the eye by intravitreal endotoxin may be locally synthesized and may be present without a substantial leukocytic infiltrate.