The two acetyl-coenzyme A synthetases of Saccharomyces cerevisiae differ with respect to kinetic properties and transcriptional regulation

• Published in: The Journal of biological chemistry Vol. 271; no. 46; pp. 28953 - 28959
• Main Authors: Berg, M.A. van den (Delft University of Technology, Delft, The Netherlands.), Jong-Gubbels, P. de, Kortland, C.J, Dijken, J.P. van, Pronk, J.T, Steensma, H.Y
• Format: Journal Article
• Language: English
• Published: United States American Society for Biochemistry and Molecular Biology 15.11.1996
• Subjects: acetate-CoA ligase; Acetate-CoA Ligase - genetics; Acetate-CoA Ligase - metabolism; acetic acid; ACIDE ACETIQUE; ACIDO ACETICO; ACTIVIDAD ENZIMATICA; ACTIVITE ENZYMATIQUE; aerobic conditions; ARN MENSAJERO; ARN MESSAGER; drug effects; enzyme activity; enzymology; ETANOL; ETHANOL; Ethanol - pharmacology; EXPRESION GENICA; EXPRESSION DES GENES; Fermentation; GENE; gene expression; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Enzymologic - drug effects; Gene Expression Regulation, Fungal; Gene Expression Regulation, Fungal - drug effects; GENES; GENETICA; genetics; GENETIQUE; GLUCOSA; GLUCOSE; Glucose - metabolism; growth & development; ISOENZIMAS; ISOENZYME; Isoenzymes; Isoenzymes - metabolism; isozymes; Kinetics; LIGASAS; LIGASE; messenger RNA; metabolism; pharmacology; RNA, Messenger; RNA, Messenger - genetics; SACCHAROMYCES CEREVISIAE; Saccharomyces cerevisiae - enzymology; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - growth & development; structural genes; TRANSCRIPCION; TRANSCRIPTION; transcription (genetics); Transcription, Genetic; Transgenes

Saccharomyces cerevisiae contains two structural genes, ACS1 and ACS2, each encoding an active acetyl-coenzyme A synthetase. Characterization of enzyme activities in cell-free extracts from strains expressing either of the two genes revealed differences in the catalytic properties of the two enzymes...