The two acetyl-coenzyme A synthetases of Saccharomyces cerevisiae differ with respect to kinetic properties and transcriptional regulation
Saccharomyces cerevisiae contains two structural genes, ACS1 and ACS2, each encoding an active acetyl-coenzyme A synthetase. Characterization of enzyme activities in cell-free extracts from strains expressing either of the two genes revealed differences in the catalytic properties of the two enzymes...
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| Published in | The Journal of biological chemistry Vol. 271; no. 46; pp. 28953 - 28959 |
|---|---|
| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
American Society for Biochemistry and Molecular Biology
15.11.1996
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| Subjects | |
| Online Access | Get full text |
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| Abstract | Saccharomyces cerevisiae contains two structural genes, ACS1 and ACS2, each encoding an active acetyl-coenzyme A synthetase. Characterization of enzyme activities in cell-free extracts from strains expressing either of the two genes revealed differences in the catalytic properties of the two enzymes. The Km for acetate of Acs1p was about 30-fold lower than that of Acs2p and Acs1p, but not Acs2p, could use propionate as a substrate. Enzyme activity measurements and mRNA analyses showed that ACS1 and ACS2 were both expressed during carbon-limited growth on glucose, ethanol, and acetate in aerobic chemostat cultures. In anaerobic glucose-limited cultures, only the ACS2 gene was expressed. Based on these facts, the products of the ACS1 and ACS2 genes were identified as the previously described "aerobic" and "non-aerobic" forms of acetyl-coenzyme A synthetase, respectively. Batch and glucose-pulse experiments revealed that transcription of ACS1 is subject to glucose repression. A mutant strain lacking Acs2p was unable to grow on glucose in batch cultures, but grew readily in aerobic glucose-limited chemostat cultures, in which the low residual glucose concentration alleviated glucose repression. Experiments in which ethanol was pulsed to aerobic ethanol-limited chemostat cultures indicated that, in addition to glucose, ethanol also repressed ACS1 transcription, although to a lesser extent. In contrast, transcription of ACS2 was slightly induced by ethanol and glucose. Absence of ACS2 prevented complete glucose repression of ACS1, indicating that ACS2 (in)directly is involved in the transcriptional regulation of ACS1 |
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| AbstractList | Saccharomyces cerevisiae contains two structural genes, ACS1 and ACS2, each encoding an active acetyl-coenzyme A synthetase. Characterization of enzyme activities in cell-free extracts from strains expressing either of the two genes revealed differences in the catalytic properties of the two enzymes. The Km for acetate of Acs1p was about 30-fold lower than that of Acs2p and Acs1p, but not Acs2p, could use propionate as a substrate. Enzyme activity measurements and mRNA analyses showed that ACS1 and ACS2 were both expressed during carbon-limited growth on glucose, ethanol, and acetate in aerobic chemostat cultures. In anaerobic glucose-limited cultures, only the ACS2 gene was expressed. Based on these facts, the products of the ACS1 and ACS2 genes were identified as the previously described "aerobic" and "non-aerobic" forms of acetyl-coenzyme A synthetase, respectively. Batch and glucose-pulse experiments revealed that transcription of ACS1 is subject to glucose repression. A mutant strain lacking Acs2p was unable to grow on glucose in batch cultures, but grew readily in aerobic glucose-limited chemostat cultures, in which the low residual glucose concentration alleviated glucose repression. Experiments in which ethanol was pulsed to aerobic ethanol-limited chemostat cultures indicated that, in addition to glucose, ethanol also repressed ACS1 transcription, although to a lesser extent. In contrast, transcription of ACS2 was slightly induced by ethanol and glucose. Absence of ACS2 prevented complete glucose repression of ACS1, indicating that ACS2 (in)directly is involved in the transcriptional regulation of ACS1 Saccharomyces cerevisiae contains two structural genes, ACS1 and ACS2, each encoding an active acetyl-coenzyme A synthetase. Characterization of enzyme activities in cell-free extracts from strains expressing either of the two genes revealed differences in the catalytic properties of the two enzymes. The K sub(m) for acetate of Acs1p was about 30-fold lower than that of Acs2p and Acs1p, but not Acs2p, could use propionate as a substrate. Enzyme activity measurements and mRNA analyses showed that ACS1 and ACS2 were both expressed during carbon-limited growth on glucose, ethanol, and acetate in aerobic chemostat cultures. In anaerobic glucose-limited cultures, only the ACS2 gene was expressed. Based on these facts, the products of the ACS1 and ACS2 genes were identified as the previously described "aerobic" and "non-aerobic" forms of acetyl-coenzyme A synthetase, respectively. Batch and glucose-pulse experiments revealed that transcription of ACS1 is subject to glucose repression. A mutant strain lacking Acs2p was unable to grow on glucose in batch cultures, but grew readily in aerobic glucose-limited chemostat cultures, in which the low residual glucose concentration alleviated glucose repression. Experiments in which ethanol was pulsed to aerobic ethanol-limited chemostat cultures indicated that, in addition to glucose, ethanol also repressed ACS1 transcription, although to a lesser extent. In contrast, transcription of ACS2 was slightly induced by ethanol and glucose. Absence of ACS2 prevented complete glucose repression of ACS1, indicating that ACS2 (in)directly is involved in the transcriptional regulation of ACS1. Saccharomyces cerevisiae contains two structural genes, ACS1 and ACS2, each encoding an active acetyl-coenzyme A synthetase. Characterization of enzyme activities in cell-free extracts from strains expressing either of the two genes revealed differences in the catalytic properties of the two enzymes. The Km for acetate of Acs1p was about 30-fold lower than that of Acs2p and Acs1p, but not Acs2p, could use propionate as a substrate. Enzyme activity measurements and mRNA analyses showed that ACS1 and ACS2 were both expressed during carbon-limited growth on glucose, ethanol, and acetate in aerobic chemostat cultures. In anaerobic glucose-limited cultures, only the ACS2 gene was expressed. Based on these facts, the products of the ACS1 and ACS2 genes were identified as the previously described "aerobic" and "non-aerobic" forms of acetyl-coenzyme A synthetase, respectively. Batch and glucose-pulse experiments revealed that transcription of ACS1 is subject to glucose repression. A mutant strain lacking Acs2p was unable to grow on glucose in batch cultures, but grew readily in aerobic glucose-limited chemostat cultures, in which the low residual glucose concentration alleviated glucose repression. Experiments in which ethanol was pulsed to aerobic ethanol-limited chemostat cultures indicated that, in addition to glucose, ethanol also repressed ACS1 transcription, although to a lesser extent. In contrast, transcription of ACS2 was slightly induced by ethanol and glucose. Absence of ACS2 prevented complete glucose repression of ACS1, indicating that ACS2 (in)directly is involved in the transcriptional regulation of ACS1.Saccharomyces cerevisiae contains two structural genes, ACS1 and ACS2, each encoding an active acetyl-coenzyme A synthetase. Characterization of enzyme activities in cell-free extracts from strains expressing either of the two genes revealed differences in the catalytic properties of the two enzymes. The Km for acetate of Acs1p was about 30-fold lower than that of Acs2p and Acs1p, but not Acs2p, could use propionate as a substrate. Enzyme activity measurements and mRNA analyses showed that ACS1 and ACS2 were both expressed during carbon-limited growth on glucose, ethanol, and acetate in aerobic chemostat cultures. In anaerobic glucose-limited cultures, only the ACS2 gene was expressed. Based on these facts, the products of the ACS1 and ACS2 genes were identified as the previously described "aerobic" and "non-aerobic" forms of acetyl-coenzyme A synthetase, respectively. Batch and glucose-pulse experiments revealed that transcription of ACS1 is subject to glucose repression. A mutant strain lacking Acs2p was unable to grow on glucose in batch cultures, but grew readily in aerobic glucose-limited chemostat cultures, in which the low residual glucose concentration alleviated glucose repression. Experiments in which ethanol was pulsed to aerobic ethanol-limited chemostat cultures indicated that, in addition to glucose, ethanol also repressed ACS1 transcription, although to a lesser extent. In contrast, transcription of ACS2 was slightly induced by ethanol and glucose. Absence of ACS2 prevented complete glucose repression of ACS1, indicating that ACS2 (in)directly is involved in the transcriptional regulation of ACS1. Saccharomyces cerevisiae contains two structural genes, ACS1 and ACS2 , each encoding an active acetyl-coenzyme A synthetase. Characterization of enzyme activities in cell-free extracts from strains expressing either of the two genes revealed differences in the catalytic properties of the two enzymes. The K m for acetate of Acs1p was about 30-fold lower than that of Acs2p and Acs1p, but not Acs2p, could use propionate as a substrate. Enzyme activity measurements and mRNA analyses showed that ACS1 and ACS2 were both expressed during carbon-limited growth on glucose, ethanol, and acetate in aerobic chemostat cultures. In anaerobic glucose-limited cultures, only the ACS2 gene was expressed. Based on these facts, the products of the ACS1 and ACS2 genes were identified as the previously described âaerobicâ and ânon-aerobicâ forms of acetyl-coenzyme A synthetase, respectively. Batch and glucose-pulse experiments revealed that transcription of ACS1 is subject to glucose repression. A mutant strain lacking Acs2p was unable to grow on glucose in batch cultures, but grew readily in aerobic glucose-limited chemostat cultures, in which the low residual glucose concentration alleviated glucose repression. Experiments in which ethanol was pulsed to aerobic ethanol-limited chemostat cultures indicated that, in addition to glucose, ethanol also repressed ACS1 transcription, although to a lesser extent. In contrast, transcription of ACS2 was slightly induced by ethanol and glucose. Absence of ACS2 prevented complete glucose repression of ACS1 , indicating that ACS2 (in)directly is involved in the transcriptional regulation of ACS1 . Saccharomyces cerevisiae contains two structural genes, ACS1 and ACS2, each encoding an active acetyl-coenzyme A synthetase. Characterization of enzyme activities in cell-free extracts from strains expressing either of the two genes revealed differences in the catalytic properties of the two enzymes. The Km for acetate of Acs1p was about 30-fold lower than that of Acs2p and Acs1p, but not Acs2p, could use propionate as a substrate. Enzyme activity measurements and mRNA analyses showed that ACS1 and ACS2 were both expressed during carbon-limited growth on glucose, ethanol, and acetate in aerobic chemostat cultures. In anaerobic glucose-limited cultures, only the ACS2 gene was expressed. Based on these facts, the products of the ACS1 and ACS2 genes were identified as the previously described "aerobic" and "non-aerobic" forms of acetyl-coenzyme A synthetase, respectively. Batch and glucose-pulse experiments revealed that transcription of ACS1 is subject to glucose repression. A mutant strain lacking Acs2p was unable to grow on glucose in batch cultures, but grew readily in aerobic glucose-limited chemostat cultures, in which the low residual glucose concentration alleviated glucose repression. Experiments in which ethanol was pulsed to aerobic ethanol-limited chemostat cultures indicated that, in addition to glucose, ethanol also repressed ACS1 transcription, although to a lesser extent. In contrast, transcription of ACS2 was slightly induced by ethanol and glucose. Absence of ACS2 prevented complete glucose repression of ACS1, indicating that ACS2 (in)directly is involved in the transcriptional regulation of ACS1. |
| Author | Dijken, J.P. van Steensma, H.Y Berg, M.A. van den (Delft University of Technology, Delft, The Netherlands.) Jong-Gubbels, P. de Kortland, C.J Pronk, J.T |
| Author_xml | – sequence: 1 fullname: Berg, M.A. van den (Delft University of Technology, Delft, The Netherlands.) – sequence: 2 fullname: Jong-Gubbels, P. de – sequence: 3 fullname: Kortland, C.J – sequence: 4 fullname: Dijken, J.P. van – sequence: 5 fullname: Pronk, J.T – sequence: 6 fullname: Steensma, H.Y |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/8910545$$D View this record in MEDLINE/PubMed |
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| Issue | 46 |
| Language | English |
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| References | Van den Berg (10.1074/jbc.271.46.28953_bib3) 1995; 231 Postma (10.1074/jbc.271.46.28953_bib26) 1989; 53 Lombardo (10.1074/jbc.271.46.28953_bib28) 1992; 12 DeVicenzi (10.1074/jbc.271.46.28953_bib9) 1970; 872 Klein (10.1074/jbc.271.46.28953_bib23) 1971; 106 Mercado (10.1074/jbc.271.46.28953_bib29) 1994; 224 Van Urk (10.1074/jbc.271.46.28953_bib27) 1990; 56 10.1074/jbc.271.46.28953_bib12 Petrik (10.1074/jbc.271.46.28953_bib5) 1983; 135 Holzer (10.1074/jbc.271.46.28953_bib1) 1957; 329 Kratzer (10.1074/jbc.271.46.28953_bib16) 1995; 161 Grundy (10.1074/jbc.271.46.28953_bib20) 1993; 10 Satyanarayana (10.1074/jbc.271.46.28953_bib13) 1976; 174 Satyanarayana (10.1074/jbc.271.46.28953_bib10) 1973; 115 Verduyn (10.1074/jbc.271.46.28953_bib7) 1991; 60 Verduyn (10.1074/jbc.271.46.28953_bib19) 1990; 136 DeVirgilio (10.1074/jbc.271.46.28953_bib15) 1992; 8 De Jong-Gubbels (10.1074/jbc.271.46.28953_bib25) 1995; 11 Sierkstra (10.1074/jbc.271.46.28953_bib24) 1992; 8 Zonneveld (10.1074/jbc.271.46.28953_bib17) 1986; 4 Verduyn (10.1074/jbc.271.46.28953_bib18) 1992; 8 Pronk (10.1074/jbc.271.46.28953_bib21) 1994; 170 Klein (10.1074/jbc.271.46.28953_bib11) 1979; 137 Frenkel (10.1074/jbc.271.46.28953_bib14) 1977; 252 Schmitt (10.1074/jbc.271.46.28953_bib22) 1990; 18 Pronk (10.1074/jbc.271.46.28953_bib2) 1994; 140 Van Urk (10.1074/jbc.271.46.28953_bib6) 1988; 4 Flikweert (10.1074/jbc.271.46.28953_bib4) 1996; 12 Satyanarayana (10.1074/jbc.271.46.28953_bib8) 1974; 341 |
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| Snippet | Saccharomyces cerevisiae contains two structural genes, ACS1 and ACS2, each encoding an active acetyl-coenzyme A synthetase. Characterization of enzyme... Saccharomyces cerevisiae contains two structural genes, ACS1 and ACS2 , each encoding an active acetyl-coenzyme A synthetase. Characterization of enzyme... |
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| SubjectTerms | acetate-CoA ligase Acetate-CoA Ligase - genetics Acetate-CoA Ligase - metabolism acetic acid ACIDE ACETIQUE ACIDO ACETICO ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE aerobic conditions ARN MENSAJERO ARN MESSAGER drug effects enzyme activity enzymology ETANOL ETHANOL Ethanol - pharmacology EXPRESION GENICA EXPRESSION DES GENES Fermentation GENE gene expression Gene Expression Regulation, Enzymologic Gene Expression Regulation, Enzymologic - drug effects Gene Expression Regulation, Fungal Gene Expression Regulation, Fungal - drug effects GENES GENETICA genetics GENETIQUE GLUCOSA GLUCOSE Glucose - metabolism growth & development ISOENZIMAS ISOENZYME Isoenzymes Isoenzymes - metabolism isozymes Kinetics LIGASAS LIGASE messenger RNA metabolism pharmacology RNA, Messenger RNA, Messenger - genetics SACCHAROMYCES CEREVISIAE Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - growth & development structural genes TRANSCRIPCION TRANSCRIPTION transcription (genetics) Transcription, Genetic Transgenes |
| Title | The two acetyl-coenzyme A synthetases of Saccharomyces cerevisiae differ with respect to kinetic properties and transcriptional regulation |
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