Influence of extremely-low-frequency magnetic field on antioxidative melatonin properties in AT478 murine squamous cell carcinoma culture

• Published in: Biological trace element research Vol. 102; no. 1-3; pp. 227 - 243
• Main Authors: Żwirska-Korczala, K, Adamczyk-Sowa, M, Polaniak, R, Sowa, P, Birkner, E, Drzazga, Z, Brzozowski, T, Konturek, S. J
• Format: Journal Article
• Language: English
• Published: United States Humana Press 2004; Springer Nature B.V
• Subjects: Animals; Antioxidants - metabolism; Cancer; Carcinoma, Squamous Cell; Cell culture; Cell Line, Tumor; culture media; Electromagnetic Fields - adverse effects; enzyme activity; Enzymes; Female; Glutathione peroxidase; Glutathione Peroxidase - metabolism; Lipid peroxidation; Magnetic fields; Malondialdehyde; Malondialdehyde - metabolism; Manganese; Melatonin; Melatonin - pharmacology; Mice; Oxidative Stress - drug effects; squamous cell carcinoma; Statistical analysis; Superoxide dismutase; Superoxide Dismutase - metabolism; Time Factors; Uterine Cervical Neoplasms
• ISSN: 0163-4984; 1559-0720; 0163-4984
• DOI: 10.1385/bter:102:1-3:227

Effects of melatonin, extremely-low-frequency magnetic field (ELF-MF), and their combination on AT478 murine squamous cell carcinoma line were studied. Manganese superoxide dismutase (MnSOD), copper-zinc superoxide dismutase (Cu/ZnSOD), and glutathione peroxidase (GSH-Px) were used as markers of cells antioxidative status, and malondialdehyde (MDA) level was used as a marker of lipid peroxidation. After melatonin treatment, antioxidative enzyme activities were increased and MDA level was decreased. Application of ELF-MF on treated cells caused an increase of both superoxide dismutases activity and MDA level, but influence of ELF-MF on GSH-Px activity was negligible. All enzyme activity in culture medium containing melatonin (10⁻³, 10⁻⁴, 10⁻⁵ M) after exposure to ELF-MF were significantly diminished compared to cells treated only with melatonin. Also MDA levels after combined treatment with melatonin and ELF-MF were significantly decreased. Observed changes were statistically significant (p<0.05). These results strongly suggest that ELF-MF attenuates antioxidative actions of melatonin on cellular level.