Resistance of the oyster pathogen Vibrio tasmaniensis LGP32 against grazing by Vannella sp. marine amoeba involves Vsm and CopA virulence factors

• Published in: Environmental microbiology Vol. 22; no. 10; pp. 4183 - 4197
• Main Authors: Robino, Etienne, Poirier, Aurore C., Amraoui, Hajar, Le Bissonnais, Sandra, Perret, Angélique, Lopez‐Joven, Carmen, Auguet, Jean‐Christophe, Rubio, Tristan P., Cazevieille, Chantal, Rolland, Jean‐Luc, Héchard, Yann, Destoumieux‐Garzón, Delphine, Charrière, Guillaume M.
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01.10.2020; Wiley Subscription Services, Inc; Society for Applied Microbiology and Wiley-Blackwell
• Subjects: Adenosine triphosphatase; Amoeba; Amoeba - physiology; Amoebozoa - physiology; Animals; Aquaculture; Bacteria; Bacterial Proteins - genetics; Biodiversity; Efflux; Farms; Grazing; Hemocytes; Immune system; Life Sciences; Marine environment; Marine molluscs; Metalloproteinase; Ostreidae - microbiology; Oysters; Pathogens; Phagocytes; Phagocytosis; Phenotypes; Populations and Evolution; Predatory Behavior; Shellfish; Vannella; Vibrio; Vibrio - genetics; Vibrio - pathogenicity; Vibrio - physiology; Virulence; Virulence factors; Virulence Factors - genetics; Waterborne diseases
• DOI: 10.1111/1462-2920.14770
• ISSN: 1462-2912

Summary Vibrios are ubiquitous in marine environments and opportunistically colonize a broad range of hosts. Strains of Vibrio tasmaniensis present in oyster farms can thrive in oysters during juvenile mortality events and behave as facultative intracellular pathogen of oyster haemocytes. Herein, we wondered whether V. tasmaniensis LGP32 resistance to phagocytosis is specific to oyster immune cells or contributes to resistance to other phagocytes, like marine amoebae. To address this question, we developed an integrative study, from the first description of amoeba diversity in oyster farms to the characterization of LGP32 interactions with amoebae. An isolate of the Vannella genus, Vannella sp. AP1411, which was collected from oyster farms, is ubiquitous, and belongs to one clade of Vannella that could be found associated with Vibrionaceae. LGP32 was shown to be resistant to grazing by Vannella sp. AP1411 and this phenotype depends on some previously identified virulence factors: secreted metalloprotease Vsm and copper efflux p‐ATPase CopA, which act at different steps during amoeba–vibrio interactions, whereas some other virulence factors were not involved. Altogether, our work indicates that some virulence factors can be involved in multi‐host interactions of V. tasmaniensis ranging from protozoans to metazoans, potentially favouring their opportunistic behaviour.