Dynamic light scattering study of the two-domain structure of Humicola insolens endoglucanase V

• Published in: FEBS letters Vol. 376; no. 1; pp. 49 - 52
• Main Authors: Boisset, Claire, Borsali, Redouane, Schülein, Martin, Henrissat, Bernard
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 27.11.1995; Wiley
• Subjects: Binding Sites; Cellulase; Cellulase - chemistry; Cloning, Molecular; Domain structure; Dynamic light scattering; Endoglucanase; Fungal Proteins - chemistry; Fungi - enzymology; Gene Expression Regulation, Fungal - genetics; Humicola insolens; Recombinant Proteins - genetics; Scattering, Radiation; Endoglucanase; Domain structure; Dynamic light scattering; Humicola insolens; Cellulase

Endoglucanase V (EG V) of Humicola insolens is composed of a catalytic domain and of a cellulose-binding domain linked by a 33 amino acid long peptide rich in Ser, Thr and Pro residues. This work describes the dynamic behavior of the two-domain structure of EG V as revealed by quasi-elastic light sc...