Efficient succinic acid production from lignocellulosic biomass by simultaneous utilization of glucose and xylose in engineered Escherichia coli

• Published in: Bioresource technology Vol. 149; pp. 84 - 91
• Main Authors: Liu, Rongming, Liang, Liya, Li, Feng, Wu, Mingke, Chen, Kequan, Ma, Jiangfeng, Jiang, Min, Wei, Ping, Ouyang, Pingkai
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.12.2013; Elsevier
• Subjects: Adenosine Triphosphate; Adenosine Triphosphate - metabolism; Anaerobiosis; ATP; Bacillus subtilis; Batch Cell Culture Techniques; Biological and medical sciences; Biomass; Bioreactors; Cellulose; Cellulose - metabolism; chemistry; Deletion; enzymology; Escherichia coli; Escherichia coli - enzymology; Escherichia coli - metabolism; Fermentation; Fundamental and applied biological sciences. Psychology; Genetic Engineering; Glucose; Glucose - metabolism; Hydrolysis; Lignin; Lignin - metabolism; Lignocellulosic hydrolysate; metabolism; Phosphoenolpyruvate Carboxylase; Phosphoenolpyruvate Carboxylase - metabolism; Phosphoenolpyruvate Sugar Phosphotransferase System; Phosphoenolpyruvate Sugar Phosphotransferase System - metabolism; Saccharum; Saccharum - chemistry; Simultaneous utilization; Strain; Succinic acid; Succinic Acid - metabolism; sugarcane bagasse; Utilization; Xylose; Xylose - metabolism; Succinic acid; Simultaneous utilization; ATP; Lignocellulosic hydrolysate; Escherichia coli; Xylose; Hydrolysate; Bacteria; Lignocellulosics; Biomass; Glucose; Enterobacteriaceae

•We constructed a recombinant Escherichia coli strain named BA305.•BA305 improved utilization of glucose and xylose anaerobically.•BA305 consumed sugar mixture simultaneously during anaerobic fermentations.•Fed-batch fermentation of sugarcane bagasse hydrolysate was achieved in BA305.•39.3 g L−1 suc...