microRNA-210 is involved in the regulation of postmenopausal osteoporosis through promotion of VEGF expression and osteoblast differentiation

• Published in: Biological chemistry Vol. 396; no. 4; pp. 339 - 347
• Main Authors: Liu, Xiao-Dong, Cai, Feng, Liu, Liang, Zhang, Yan, Yang, An-Li
• Format: Journal Article
• Language: English
• Published: Germany De Gruyter 01.04.2015
• Subjects: Adipocytes - cytology; Adipocytes - metabolism; Animals; BMSC differentiation; Cell Differentiation; Cells, Cultured; Estrogens - metabolism; Female; Humans; Hypoxia-Inducible Factor 1, alpha Subunit - genetics; Mesenchymal Stromal Cells - cytology; Mesenchymal Stromal Cells - metabolism; MicroRNAs - metabolism; miR-210; Osteoblasts - cytology; Osteoblasts - metabolism; Osteogenesis; Osteoporosis, Postmenopausal - genetics; Osteoporosis, Postmenopausal - metabolism; Phosphatidylinositol 3-Kinases - metabolism; postmenopausal osteoporosis; Rats; Rats, Wistar; Signal Transduction; Vascular Endothelial Growth Factor A - genetics; VEGF
• DOI: 10.1515/hsz-2014-0268
• ISSN: 1431-6730; 1437-4315

MicroRNAs (miRNAs) are small non-protein-codingRNAs that function as negative gene expression regulators. miRNA-210 (miR-210) has recently been recognized in the pathogenesis of osteonecrosis associated with angiogenesis. Herein we aimed to explore the clinical significance of miR-210 treatment for postmenopausal osteoporosis. The expression of miR-210 was detected in bone marrow mesenchymal stem cells (BMSCs) and miR-210 significantly promoted the expression of vascular edothelial growth factor (VEGF) in BMSCs in a time-dependent manner ( <0.05). And miR-210 suppressed PPARγ expression but increased the expression of ALP and osterix, demonstrating that miR-210 inhibited adipocyte differentiation and promoted osteoblast differentiation of BMSCs . The protein expression of hypoxia-inducible factor 1 alpha (HIF-1α) and VEGF in 17β-estradiol (E2) treated osteoblasts were significantly increased in a dose- and time-dependent manner ( <0.05). And E2 inducted the VEGF expression through the PI3K/AKT signaling pathway in osteoblasts. Taken together, these data implied that miR-210 played an important role in ameliorating the estrogen deficiency caused-postmenopausal osteoporosis through promotion the VEGF expression and osteoblast differentiation.