Retrieval, Maturation, and Fertilization of Immature Oocytes Obtained from Unstimulated Patients with Polycystic Ovary Syndrome

• Published in: Journal of assisted reproduction and genetics Vol. 16; no. 2; pp. 81 - 86
• Main Authors: Beckers, Nicole G. M., Pieters, Math H. E. C., Ramos, Liliana, Zeilmaker, Gerard H., Fauser, Bart C. J. M., Braat, Didi D. M.
• Format: Journal Article
• Language: English
• Published: New York, NY Kluwer/Plenum 01.02.1999; Springer Nature B.V; Kluwer Academic Publishers-Plenum Publishers
• Subjects: Adult; Biological and medical sciences; Cellular Senescence - physiology; Embryo Transfer; Female; Female genital diseases; Fertilization in Vitro; Gynecology. Andrology. Obstetrics; Humans; Medical sciences; Oocyte Donation; Pilot Projects; Polycystic Ovary Syndrome - etiology; Polycystic Ovary Syndrome - therapy; Stimulation, Chemical; Tumors; Estrogen; Female sterility; Gonadotropin; Polycystic ovary; Germinal cell; Fecundation; Estradiol; In vitro; Ovarian hormone; Female genital diseases; Ovarian diseases; Adenohypophyseal hormone; Uterus; Female genital system; Cyst; Luteinizing hormone; Female; Benign neoplasm; Sex steroid hormone; Immaturity; Endometrium; Oocyte; Follicle stimulating hormone; Oocyte maturation
• ISSN: 1058-0468; 1573-7330
• DOI: 10.1023/A:1022516806423

Our purpose was to determine whether immature oocytes could be retrieved under local anesthesia, whether these oocytes would mature and fertilize in vitro, and whether adequate endometrium development could be obtained after hormonal supplementation. Ovum pick-up was performed under local anesthesia. Immature oocytes were cultured and inseminated. To prepare the endometrium, estradiolvalerate was administered in combination with micronized progesterone. Immature oocytes were obtained in all cases. Fifty-six percent (n = 30) of the oocytes developed into metaphase II (MII) after 48 hr of culture, and another 20% reached the MII stage by 72 hr. Normal fertilization was observed in only 10% of oocytes inseminated. No embryonic development occurred, and therefore embryo transfer was not performed in any of the patients. Endometrial microbiopsy was performed in all subjects and endometrial development was considered sufficient in eight patients. We collected immature oocytes from patients with polycystic ovary syndrome without general anesthesia. In vitro maturation of these oocytes seemed adequate but fertilization rates were poor. Sufficient endometrial quality was obtained after hormonal substitution.