Stoichiometric Assembly of the Cellulosome Generates Maximum Synergy for the Degradation of Crystalline Cellulose, as Revealed by In Vitro Reconstitution of the Clostridium thermocellum Cellulosome

• Published in: Applied and Environmental Microbiology Vol. 81; no. 14; pp. 4756 - 4766
• Main Authors: Hirano, Katsuaki, Nihei, Satoshi, Hasegawa, Hiroki, Haruki, Mitsuru, Hirano, Nobutaka
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 01.07.2015
• Subjects: Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Cellulases - genetics; Cellulases - metabolism; Cellulose; Cellulose - chemistry; Cellulose - metabolism; Cellulosomes - enzymology; Cellulosomes - genetics; Cellulosomes - metabolism; Chromatography; Clostridium thermocellum; Clostridium thermocellum - enzymology; Clostridium thermocellum - genetics; Clostridium thermocellum - metabolism; Crystallization; Enzymes; Enzymology and Protein Engineering; Gram-positive bacteria; Molecular chemistry; Protein expression; Protein synthesis; Spotlight; Triticum aestivum

The cellulosome is a supramolecular multienzyme complex formed by species-specific interactions between the cohesin modules of scaffoldin proteins and the dockerin modules of a wide variety of polysaccharide-degrading enzymes. Cellulosomal enzymes bound to the scaffoldin protein act synergistically...