Optimized methods to image hepatic lipid droplets in zebrafish larvae

• Published in: Disease models & mechanisms Vol. 17; no. 11
• Main Authors: Khan, Nouf, Mohd Salmi, Talhah, Karamalakis, Anthony P., Ramdas Nair, Anjana, Sadler, Kirsten C., Cox, Andrew G.
• Format: Journal Article
• Language: English
• Published: England The Company of Biologists Ltd 01.11.2024; The Company of Biologists
• Subjects: Animals; Animals, Genetically Modified; Azo Compounds; Green Fluorescent Proteins - metabolism; Larva - metabolism; lipid droplet; Lipid Droplets - metabolism; Liver - metabolism; Liver - pathology; liver disease; Oxazines - metabolism; Perilipin-2 - metabolism; Resources & Methods; steatosis; zebrafish; Zebrafish - metabolism; Zebrafish Proteins - metabolism; Zebrafish; Lipid droplet; Liver disease; Steatosis
• ISSN: 1754-8403; 1754-8411; 1754-8411
• DOI: 10.1242/dmm.050786

The optical transparency of zebrafish larvae enables visualization of subcellular structures in intact organs, and these vertebrates are widely used to study lipid biology and liver disease. Lipid droplet (LD) presence is a prevalent feature of healthy cells, but, under conditions such as nutrient e...