Characterization of a Williopsis saturnus var. mrakii high molecular weight secreted killer toxin with broad-spectrum antimicrobial activity

• Published in: Journal of antimicrobial chemotherapy Vol. 49; no. 6; pp. 961 - 971
• Main Authors: Guyard, Cyril, Séguy, Nathalie, Cailliez, Jean-Charles, Drobecq, Hervé, Polonelli, Luciano, Dei-Cas, Eduardo, Mercenier, Annick, Menozzi, Franco D.
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01.06.2002; Oxford Publishing Limited (England)
• Subjects: Amino Acid Sequence - genetics; Anti-Infective Agents - chemistry; Anti-Infective Agents - isolation & purification; Anti-Infective Agents - pharmacology; Antibiotics. Antiinfectious agents. Antiparasitic agents; Antifungal agents; Biological and medical sciences; Fungal Proteins - chemistry; Fungal Proteins - genetics; Fungal Proteins - metabolism; Fungal Proteins - pharmacology; Killer Factors, Yeast; Medical sciences; Molecular Sequence Data; Molecular Weight; Mycotoxins - chemistry; Mycotoxins - genetics; Mycotoxins - metabolism; Mycotoxins - pharmacology; Pharmacology. Drug treatments; Saccharomyces cerevisiae - drug effects; Saccharomyces cerevisiae Proteins; Yeast; Pneumocystis carinii; Glycosylation; Biological activity; Cell wall; Protein; Pichia; Fungi; Toxin; Antifungal agent; Ascomycetes; Fungi Imperfecti; Saccharomyces cerevisiae; Aminoacid sequence; Thallophyta

Williopsis saturnus var. mrakii MUCL 41968 secretes a killer toxin (WmKT), which is active against a wide range of pathogens. From the W. saturnus var. mrakii MUCL 41968 culture supernatant a protein of 85 kDa with killer activity was purified to homogeneity. The purified protein was demonstrated to...