Functional RNA microarrays for high-throughput screening of antiprotein aptamers

High-throughput methods for generating aptamer microarrays are described. As a proof-of-principle, the microarrays were used to screen the affinity and specificity of a pool of robotically selected antilysozyme RNA aptamers. Aptamers were transcribed in vitro in reactions supplemented with biotinyl-...

Full description

Saved in:
Bibliographic Details
Published inAnalytical biochemistry Vol. 338; no. 1; pp. 113 - 123
Main Authors Collett, James R., Cho, Eun Jeong, Lee, Jennifer F., Levy, Matthew, Hood, Allysia J., Wan, Christine, Ellington, Andrew D.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.03.2005
Subjects
Online AccessGet full text

Cover

Loading…
Abstract High-throughput methods for generating aptamer microarrays are described. As a proof-of-principle, the microarrays were used to screen the affinity and specificity of a pool of robotically selected antilysozyme RNA aptamers. Aptamers were transcribed in vitro in reactions supplemented with biotinyl-guanosine 5′-monophosphate, which led to the specific addition of a 5′ biotin moiety, and then spotted on streptavidin-coated microarray slides. The aptamers captured target protein in a dose-dependent manner, with linear signal response ranges that covered seven orders of magnitude and a lower limit of detection of 1 pg/mL (70 fM). Aptamers on the microarray retained their specificity for target protein in the presence of a 10,000-fold (w/w) excess of T-4 cell lysate protein. The RNA aptamer microarrays performed comparably to current antibody microarrays and within the clinically relevant ranges of many disease biomarkers. These methods should also prove useful for generating other functional RNA microarrays, including arrays for genomic noncoding RNAs that bind proteins. Integrating RNA aptamer microarray production with the maturing technology for automated in vitro selection of antiprotein aptamers should result in the high-throughput production of proteome chips.
AbstractList High-throughput methods for generating aptamer microarrays are described. As a proof-of-principle, the microarrays were used to screen the affinity and specificity of a pool of robotically selected antilysozyme RNA aptamers. Aptamers were transcribed in vitro in reactions supplemented with biotinyl-guanosine 5'-monophosphate, which led to the specific addition of a 5' biotin moiety, and then spotted on streptavidin-coated microarray slides. The aptamers captured target protein in a dose-dependent manner, with linear signal response ranges that covered seven orders of magnitude and a lower limit of detection of 1 pg/mL (70 fM). Aptamers on the microarray retained their specificity for target protein in the presence of a 10,000-fold (w/w) excess of T-4 cell lysate protein. The RNA aptamer microarrays performed comparably to current antibody microarrays and within the clinically relevant ranges of many disease biomarkers. These methods should also prove useful for generating other functional RNA microarrays, including arrays for genomic noncoding RNAs that bind proteins. Integrating RNA aptamer microarray production with the maturing technology for automated in vitro selection of antiprotein aptamers should result in the high-throughput production of proteome chips.
High-throughput methods for generating aptamer microarrays are described. As a proof-of-principle, the microarrays were used to screen the affinity and specificity of a pool of robotically selected antilysozyme RNA aptamers. Aptamers were transcribed in vitro in reactions supplemented with biotinyl-guanosine 5′-monophosphate, which led to the specific addition of a 5′ biotin moiety, and then spotted on streptavidin-coated microarray slides. The aptamers captured target protein in a dose-dependent manner, with linear signal response ranges that covered seven orders of magnitude and a lower limit of detection of 1 pg/mL (70 fM). Aptamers on the microarray retained their specificity for target protein in the presence of a 10,000-fold (w/w) excess of T-4 cell lysate protein. The RNA aptamer microarrays performed comparably to current antibody microarrays and within the clinically relevant ranges of many disease biomarkers. These methods should also prove useful for generating other functional RNA microarrays, including arrays for genomic noncoding RNAs that bind proteins. Integrating RNA aptamer microarray production with the maturing technology for automated in vitro selection of antiprotein aptamers should result in the high-throughput production of proteome chips.
Author Levy, Matthew
Wan, Christine
Lee, Jennifer F.
Collett, James R.
Hood, Allysia J.
Cho, Eun Jeong
Ellington, Andrew D.
Author_xml – sequence: 1
  givenname: James R.
  surname: Collett
  fullname: Collett, James R.
– sequence: 2
  givenname: Eun Jeong
  surname: Cho
  fullname: Cho, Eun Jeong
– sequence: 3
  givenname: Jennifer F.
  surname: Lee
  fullname: Lee, Jennifer F.
– sequence: 4
  givenname: Matthew
  surname: Levy
  fullname: Levy, Matthew
– sequence: 5
  givenname: Allysia J.
  surname: Hood
  fullname: Hood, Allysia J.
– sequence: 6
  givenname: Christine
  surname: Wan
  fullname: Wan, Christine
– sequence: 7
  givenname: Andrew D.
  surname: Ellington
  fullname: Ellington, Andrew D.
  email: andy.ellington@mail.utexas.edu
BackLink https://www.ncbi.nlm.nih.gov/pubmed/15707941$$D View this record in MEDLINE/PubMed
BookMark eNqFkM-L1EAQhRtZcWdX754kJ2-JVUn_SLwti6vCoiJ6biqdykwPk87Y3RH2vzfDDHgST3X53uPVdyOuwhxYiNcIFQLqd_uK-qoGkBViBbV5JjYInS6hge5KbACgKWvdmWtxk9IeAFEq_UJcozJgOokb8e1hCS77OdCh-P7lrpi8izPFSE-pGOdY7Px2V-ZdnJft7rjkIrnIHHzYFvNYUMj-GOfMPhR0zDRxTC_F85EOiV9d7q34-fDhx_2n8vHrx8_3d4-lk1Llsm-dHtYRjanBaVKoDQ9tL7lvFCO5bhylkp2WAOP6HxkkVESKtAGqWTW34u25dx3wa-GU7eST48OBAs9LstrIumk7-C-IpgVo1QmEM7gaSCnyaI_RTxSfLII96bZ7S7096baIdtW9Rt5cupd-4uFv4OJ3Bd6fAV5V_PYcbXKeg-PBR3bZDrP_d_sf-mCQ8w
CitedBy_id crossref_primary_10_1002_chem_201001192
crossref_primary_10_1093_nar_gkn775
crossref_primary_10_1016_j_biotechadv_2013_04_007
crossref_primary_10_1093_nar_gkm922
crossref_primary_10_1016_j_sbi_2014_01_014
crossref_primary_10_2144_000112187
crossref_primary_10_1016_j_aca_2005_12_038
crossref_primary_10_1021_ac400746j
crossref_primary_10_3390_molecules19044986
crossref_primary_10_1016_j_bios_2010_07_033
crossref_primary_10_1021_ac0516180
crossref_primary_10_1080_02648725_2007_10648106
crossref_primary_10_1246_cl_2010_454
crossref_primary_10_1016_j_ab_2018_10_010
crossref_primary_10_1016_j_ymeth_2005_05_009
crossref_primary_10_1093_nar_gkr609
crossref_primary_10_1002_jssc_200600483
crossref_primary_10_1063_1_4894851
crossref_primary_10_1002_cite_200800053
crossref_primary_10_1517_17460441_2011_537321
crossref_primary_10_1093_nar_gkp408
crossref_primary_10_1093_nar_gkr464
crossref_primary_10_1016_j_ijbiomac_2016_05_014
crossref_primary_10_1016_j_semcdb_2009_01_015
crossref_primary_10_3920_WMJ2011_1318
crossref_primary_10_3390_microarrays4020115
crossref_primary_10_1016_j_ab_2008_01_005
crossref_primary_10_1016_j_snb_2020_128073
crossref_primary_10_1039_b717527b
crossref_primary_10_1016_j_bios_2014_07_050
crossref_primary_10_1021_ac7015954
crossref_primary_10_1016_j_csbj_2019_06_004
crossref_primary_10_1016_j_bios_2006_01_003
crossref_primary_10_1016_j_bpc_2024_107218
crossref_primary_10_1002_elan_201900736
crossref_primary_10_1016_j_molmed_2009_06_004
crossref_primary_10_1002_elsc_201100100
crossref_primary_10_1021_ac801081v
crossref_primary_10_1016_j_bios_2011_02_031
crossref_primary_10_1134_S1990750807030043
crossref_primary_10_1021_ja0542946
crossref_primary_10_1016_j_snb_2016_07_142
crossref_primary_10_1093_nar_gni128
crossref_primary_10_1586_14789450_2_4_499
crossref_primary_10_1021_ja908035g
crossref_primary_10_1038_nprot_2008_185
crossref_primary_10_1039_b615477h
crossref_primary_10_1039_C3AN01880F
crossref_primary_10_1021_ac801285a
crossref_primary_10_1021_ac061849m
crossref_primary_10_1517_13543784_17_1_43
crossref_primary_10_1021_cr030183i
crossref_primary_10_1080_08927020600647052
crossref_primary_10_1002_elan_200603593
crossref_primary_10_1155_2016_9718612
crossref_primary_10_1038_srep21285
crossref_primary_10_1002_pmic_200700155
crossref_primary_10_1016_j_biochi_2018_09_001
crossref_primary_10_1021_cr0306743
crossref_primary_10_1021_la060223o
Cites_doi 10.1038/nrg1321
10.1073/pnas.0831159100
10.1016/0378-1119(92)90309-D
10.1006/mthe.2002.0624
10.1038/332469a0
10.1016/S1471-4914(02)02352-3
10.1002/pmic.200390009
10.1016/S0021-9258(18)31598-9
10.1002/pmic.200390038
10.1074/jbc.M104651200
10.1016/S0958-1669(02)00278-1
10.1016/S0003-2697(03)00297-5
10.1038/ng1037
10.1093/clinchem/45.9.1628
10.1089/aid.1987.3.283
10.1007/s00216-001-1189-3
10.1038/nbt910
10.1016/j.cccn.2004.01.009
10.1006/abio.1999.4063
10.1016/S0968-0896(01)00028-1
10.1093/nar/24.6.1029
10.1038/4439
10.1038/nbt0302-225
10.1021/bp980097h
10.1073/pnas.112683499
10.2144/dec02kusnezow
10.1002/1615-9861(200101)1:1<13::AID-PROT13>3.0.CO;2-J
10.1002/jmr.625
10.1016/S1367-5931(02)00005-4
10.1093/nar/25.17.3389
10.1023/A:1026401917416
10.1002/1097-0142(196504)18:4<522::AID-CNCR2820180418>3.0.CO;2-J
10.1126/science.289.5485.1760
10.1038/ng766
10.1074/jbc.273.23.14309
10.1093/nar/gkh094
10.1002/pmic.200300631
ContentType Journal Article
Copyright 2004 Elsevier Inc.
Copyright_xml – notice: 2004 Elsevier Inc.
DBID CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
CITATION
7TM
7X8
DOI 10.1016/j.ab.2004.11.027
DatabaseName Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
CrossRef
Nucleic Acids Abstracts
MEDLINE - Academic
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
CrossRef
Nucleic Acids Abstracts
MEDLINE - Academic
DatabaseTitleList MEDLINE

Nucleic Acids Abstracts
MEDLINE - Academic
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
DeliveryMethod fulltext_linktorsrc
Discipline Anatomy & Physiology
Chemistry
EISSN 1096-0309
EndPage 123
ExternalDocumentID 10_1016_j_ab_2004_11_027
15707941
S0003269704009315
Genre Research Support, U.S. Gov't, Non-P.H.S
Research Support, U.S. Gov't, P.H.S
Research Support, Non-U.S. Gov't
Journal Article
Research Support, N.I.H., Extramural
GrantInformation_xml – fundername: NIBIB NIH HHS
  grantid: 8R01EB002043
GroupedDBID ---
--K
--M
-~X
.55
.GJ
.~1
0R~
1B1
1RT
1~.
1~5
23M
4.4
457
4G.
53G
5GY
5VS
6J9
7-5
71M
85S
8P~
9JM
9JN
AABNK
AACTN
AAEDT
AAEDW
AAIAV
AAIKJ
AAKOC
AALRI
AAOAW
AAQFI
AAQXK
AARLI
AAXUO
ABEFU
ABFNM
ABFRF
ABGSF
ABMAC
ABOCM
ABTAH
ABUDA
ABXDB
ABYKQ
ACDAQ
ACGFO
ACKIV
ACNCT
ACNNM
ACRLP
ADBBV
ADECG
ADEZE
ADFGL
ADIYS
ADMUD
ADRHT
ADUVX
AEBSH
AEFWE
AEHWI
AEKER
AENEX
AFKWA
AFTJW
AFXIZ
AFZHZ
AGHFR
AGRDE
AGUBO
AGYEJ
AHHHB
AHPSJ
AI.
AIEXJ
AIKHN
AITUG
AJBFU
AJOXV
AJSZI
ALMA_UNASSIGNED_HOLDINGS
AMFUW
AMRAJ
ASPBG
AVWKF
AXJTR
AZFZN
BKOJK
BLXMC
CAG
COF
CS3
DM4
DOVZS
EBS
EFBJH
EFLBG
EJD
EO8
EO9
EP2
EP3
F5P
FA8
FDB
FEDTE
FGOYB
FIRID
FLBIZ
FNPLU
FYGXN
G-2
G-Q
G8K
GBLVA
HLW
HVGLF
HZ~
H~9
IHE
J1W
J5H
K-O
KOM
L7B
LG5
LX2
M41
MO0
MVM
N9A
O-L
O9-
OAUVE
OZT
P-8
P-9
P2P
PC.
Q38
R2-
RIG
RNS
ROL
RPZ
SBG
SCB
SCC
SDF
SDG
SDP
SES
SEW
SPC
SPCBC
SSK
SSU
SSZ
T5K
VH1
WH7
WUQ
X7M
XFK
XOL
XPP
Y6R
YYP
ZA5
ZGI
ZKB
ZMT
ZY4
AAHBH
AAXKI
AKRWK
CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
ADVLN
AFJKZ
CITATION
7TM
7X8
ID FETCH-LOGICAL-c445t-b8c6d7073720c6a5167ed8b4eb35e1ac9ff45496400f004a71a15aa5a670a2e53
IEDL.DBID .~1
ISSN 0003-2697
IngestDate Fri Oct 25 09:15:33 EDT 2024
Fri Oct 25 04:08:50 EDT 2024
Thu Sep 26 17:44:23 EDT 2024
Sat Sep 28 07:52:47 EDT 2024
Fri Feb 23 02:32:40 EST 2024
IsPeerReviewed true
IsScholarly true
Issue 1
Keywords RNA aptamer
High-throughput screening
Microarray
Functional RNA
Protein detection
Proteomics
Language English
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c445t-b8c6d7073720c6a5167ed8b4eb35e1ac9ff45496400f004a71a15aa5a670a2e53
Notes ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
ObjectType-Feature-2
PMID 15707941
PQID 17800850
PQPubID 23462
PageCount 11
ParticipantIDs proquest_miscellaneous_67423890
proquest_miscellaneous_17800850
crossref_primary_10_1016_j_ab_2004_11_027
pubmed_primary_15707941
elsevier_sciencedirect_doi_10_1016_j_ab_2004_11_027
PublicationCentury 2000
PublicationDate 2005-03-01
PublicationDateYYYYMMDD 2005-03-01
PublicationDate_xml – month: 03
  year: 2005
  text: 2005-03-01
  day: 01
PublicationDecade 2000
PublicationPlace United States
PublicationPlace_xml – name: United States
PublicationTitle Analytical biochemistry
PublicationTitleAlternate Anal Biochem
PublicationYear 2005
Publisher Elsevier Inc
Publisher_xml – name: Elsevier Inc
References Kirby, Cho, Bayer, Gehrke, Park, Neikirk, McDevitt, Ellington (bib40) 2003; 75
Mattick (bib43) 2004; 5
Michaud, Salcius, Zhou, Bangham, Bonin, Guo, Snyder, Predki, Schweitzer (bib34) 2003; 21
Hicke, Marion, Chang, Gould, Lynott, Parma, Schmidt, Warren (bib17) 2001; 276
Foley, Lazarus, Farber, Uzman, Boone, McCarthy (bib24) 1965; 18
Martell, Nevins, Sullenger (bib19) 2002; 6
Szymanski, Barciszewski (bib44) 2002; 3
Jenkins, Pennington (bib8) 2001; 1
Cox, Rudolph, Ellington (bib14) 1998; 14
Hirao, Spingola, Peabody, Ellington (bib16) 1998; 4
Kusnezow, Hoheisel (bib37) 2003; 16
Pitulle, Kleineidam, Sproat, Krupp (bib29) 1992; 112
White, Shan, Rusconi, Shetty, Dewhirst, Kontos, Sullenger (bib20) 2003; 100
O’Sullivan (bib38) 2002; 372
Conrad, Keranen, Ellington, Newton (bib15) 1994; 269
Bell, Denu, Dixon, Ellington (bib21) 1998; 273
Zhu, Snyder (bib3) 2003; 7
J. DeRisi, V. Iyer, P.O. Brown, The MGuide: A Complete Guide to Building your own Microarrayer, Biochemistry Department, Stanford University, 1999
Nara, Hatch, Dunlop, Robey, Arthur, Gonda, Fischinger (bib26) 1987; 3
Kusnezow, Hoheisel (bib1) 2002
Schweitzer, Kingsmore (bib6) 2002; 13
Pavlickova, Schneider, Hug (bib31) 2004; 343
MacBeath (bib2) 2002; 32
Hesselberth, Robertson, Jhaveri, Ellington (bib11) 2000; 74
Bailey, Elkan (bib23) 1994; 2
Mitchell (bib35) 2002; 20
Jin, Riley, Wolfinger, White, Passador-Gurgel, Gibson (bib36) 2001; 29
Cheung, Morley, Aguilar, Massimi, Kucherlapati, Childs (bib32) 1999; 21
McCauley, Hamaguchi, Stanton (bib28) 2003; 319
Eickhoff, Konthur, Lueking, Lehrach, Walter, Nordhoff, Nyarsik, Bussow (bib7) 2002; 77
Brody, Gold (bib10) 2000; 74
Jayasena (bib12) 1999; 45
Cox, Ellington (bib13) 2001; 9
Altschul, Madden, Schaffer, Zhang, Zhang, Miller, Lipman (bib22) 1997; 25
Nara, Fischinger (bib25) 1988; 332
Dudley, Aach, Steffen, Church (bib33) 2002; 99
Walter, Bussow, Lueking, Glokler (bib39) 2002; 8
Sreekumar, Nyati, Varambally, Barrette, Ghosh, Lawrence, Chinnaiyan (bib5) 2001; 61
Kusnezow, Jacob, Walijew, Diehl, Hoheisel (bib41) 2003; 3
Miller, Zhou, Kwekel, Cavallo, Burke, Butler, Teh, Haab (bib4) 2003; 3
Lueking, Horn, Eickhoff, Bussow, Lehrach, Walter (bib9) 1999; 270
Bock, Coleman, Collins, Davis, Foulds, Gold, Greef, Heil, Heilig, Hicke, Hurst, Husar, Miller, Ostroff, Petach, Schneider, Vant-Hull, Waugh, Weiss, Wilcox, Zichi (bib42) 2004; 4
Geiger, Burgstaller, von der Eltz, Roeder, Famulok (bib18) 1996; 24
Lee, Hesselberth, Meyers, Ellington (bib45) 2004; 32
MacBeath, Schreiber (bib30) 2000; 289
White (10.1016/j.ab.2004.11.027_bib20) 2003; 100
Kirby (10.1016/j.ab.2004.11.027_bib40) 2003; 75
Mattick (10.1016/j.ab.2004.11.027_bib43) 2004; 5
Hesselberth (10.1016/j.ab.2004.11.027_bib11) 2000; 74
Cox (10.1016/j.ab.2004.11.027_bib13) 2001; 9
Bell (10.1016/j.ab.2004.11.027_bib21) 1998; 273
Cheung (10.1016/j.ab.2004.11.027_bib32) 1999; 21
Cox (10.1016/j.ab.2004.11.027_bib14) 1998; 14
Conrad (10.1016/j.ab.2004.11.027_bib15) 1994; 269
Szymanski (10.1016/j.ab.2004.11.027_bib44) 2002; 3
Miller (10.1016/j.ab.2004.11.027_bib4) 2003; 3
Martell (10.1016/j.ab.2004.11.027_bib19) 2002; 6
Sreekumar (10.1016/j.ab.2004.11.027_bib5) 2001; 61
Geiger (10.1016/j.ab.2004.11.027_bib18) 1996; 24
MacBeath (10.1016/j.ab.2004.11.027_bib30) 2000; 289
Lueking (10.1016/j.ab.2004.11.027_bib9) 1999; 270
Dudley (10.1016/j.ab.2004.11.027_bib33) 2002; 99
Brody (10.1016/j.ab.2004.11.027_bib10) 2000; 74
Eickhoff (10.1016/j.ab.2004.11.027_bib7) 2002; 77
Michaud (10.1016/j.ab.2004.11.027_bib34) 2003; 21
Kusnezow (10.1016/j.ab.2004.11.027_bib1) 2002
Altschul (10.1016/j.ab.2004.11.027_bib22) 1997; 25
Schweitzer (10.1016/j.ab.2004.11.027_bib6) 2002; 13
Jayasena (10.1016/j.ab.2004.11.027_bib12) 1999; 45
Nara (10.1016/j.ab.2004.11.027_bib26) 1987; 3
Kusnezow (10.1016/j.ab.2004.11.027_bib37) 2003; 16
Mitchell (10.1016/j.ab.2004.11.027_bib35) 2002; 20
Zhu (10.1016/j.ab.2004.11.027_bib3) 2003; 7
Hirao (10.1016/j.ab.2004.11.027_bib16) 1998; 4
Jenkins (10.1016/j.ab.2004.11.027_bib8) 2001; 1
Jin (10.1016/j.ab.2004.11.027_bib36) 2001; 29
Pitulle (10.1016/j.ab.2004.11.027_bib29) 1992; 112
O’Sullivan (10.1016/j.ab.2004.11.027_bib38) 2002; 372
Hicke (10.1016/j.ab.2004.11.027_bib17) 2001; 276
Kusnezow (10.1016/j.ab.2004.11.027_bib41) 2003; 3
Foley (10.1016/j.ab.2004.11.027_bib24) 1965; 18
MacBeath (10.1016/j.ab.2004.11.027_bib2) 2002; 32
McCauley (10.1016/j.ab.2004.11.027_bib28) 2003; 319
Nara (10.1016/j.ab.2004.11.027_bib25) 1988; 332
Pavlickova (10.1016/j.ab.2004.11.027_bib31) 2004; 343
Lee (10.1016/j.ab.2004.11.027_bib45) 2004; 32
Bailey (10.1016/j.ab.2004.11.027_bib23) 1994; 2
Walter (10.1016/j.ab.2004.11.027_bib39) 2002; 8
10.1016/j.ab.2004.11.027_bib27
Bock (10.1016/j.ab.2004.11.027_bib42) 2004; 4
References_xml – volume: 21
  start-page: 15
  year: 1999
  end-page: 19
  ident: bib32
  article-title: Making and reading microarrays
  publication-title: Nat. Genet
  contributor:
    fullname: Childs
– volume: 25
  start-page: 3389
  year: 1997
  end-page: 3402
  ident: bib22
  article-title: Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
  publication-title: Nucleic Acids Res.
  contributor:
    fullname: Lipman
– volume: 3
  start-page: 254
  year: 2003
  end-page: 264
  ident: bib41
  article-title: Antibody microarrays: an evaluation of production parameters
  publication-title: Proteomics
  contributor:
    fullname: Hoheisel
– volume: 29
  start-page: 389
  year: 2001
  end-page: 395
  ident: bib36
  article-title: The contributions of sex, genotype and age to transcriptional variance in
  publication-title: Nat. Genet.
  contributor:
    fullname: Gibson
– volume: 32
  start-page: D95
  year: 2004
  end-page: D100
  ident: bib45
  article-title: Aptamer database
  publication-title: Nucleic Acids Res.
  contributor:
    fullname: Ellington
– volume: 18
  start-page: 522
  year: 1965
  end-page: 529
  ident: bib24
  article-title: Continuous culture of human lymphoblasts from peripheral blood of a child with acute leukemia
  publication-title: Cancer
  contributor:
    fullname: McCarthy
– volume: 289
  start-page: 1760
  year: 2000
  end-page: 1763
  ident: bib30
  article-title: Printing proteins as microarrays for high-throughput function determination
  publication-title: Science
  contributor:
    fullname: Schreiber
– volume: 14
  start-page: 845
  year: 1998
  end-page: 850
  ident: bib14
  article-title: Automated RNA selection
  publication-title: Biotechnol. Prog.
  contributor:
    fullname: Ellington
– volume: 332
  start-page: 469
  year: 1988
  end-page: 470
  ident: bib25
  article-title: Quantitative infectivity assay for HIV-1 and-2
  publication-title: Nature
  contributor:
    fullname: Fischinger
– volume: 3
  start-page: 56
  year: 2003
  end-page: 63
  ident: bib4
  article-title: Antibody microarray profiling of human prostate cancer sera: antibody screening and identification of potential biomarkers
  publication-title: Proteomics
  contributor:
    fullname: Haab
– volume: 270
  start-page: 103
  year: 1999
  end-page: 111
  ident: bib9
  article-title: Protein microarrays for gene expression and antibody screening
  publication-title: Anal. Biochem.
  contributor:
    fullname: Walter
– volume: 74
  start-page: 15
  year: 2000
  end-page: 25
  ident: bib11
  article-title: In vitro selection of nucleic acids for diagnostic applications
  publication-title: J. Biotechnol.
  contributor:
    fullname: Ellington
– volume: 9
  start-page: 2525
  year: 2001
  end-page: 2531
  ident: bib13
  article-title: Automated selection of anti-protein aptamers
  publication-title: Bioorg. Med. Chem.
  contributor:
    fullname: Ellington
– volume: 24
  start-page: 1029
  year: 1996
  end-page: 1036
  ident: bib18
  article-title: RNA aptamers that bind
  publication-title: Nucleic Acids Res.
  contributor:
    fullname: Famulok
– volume: 269
  start-page: 32051
  year: 1994
  end-page: 32054
  ident: bib15
  article-title: Isozyme-specific inhibition of protein kinase C by RNA aptamers
  publication-title: J. Biol. Chem.
  contributor:
    fullname: Newton
– volume: 8
  start-page: 250
  year: 2002
  end-page: 253
  ident: bib39
  article-title: High-throughput protein arrays: prospects for molecular diagnostics
  publication-title: Trends Mol. Med.
  contributor:
    fullname: Glokler
– volume: 319
  start-page: 244
  year: 2003
  end-page: 250
  ident: bib28
  article-title: Aptamer-based biosensor arrays for detection and quantification of biological macromolecules
  publication-title: Anal. Biochem.
  contributor:
    fullname: Stanton
– volume: 20
  start-page: 225
  year: 2002
  end-page: 229
  ident: bib35
  article-title: A perspective on protein microarrays
  publication-title: Nat. Biotechnol.
  contributor:
    fullname: Mitchell
– volume: 5
  start-page: 316
  year: 2004
  end-page: 323
  ident: bib43
  article-title: RNA regulation: a new genetics?
  publication-title: Nat. Rev. Genet
  contributor:
    fullname: Mattick
– volume: 100
  start-page: 5028
  year: 2003
  end-page: 5033
  ident: bib20
  article-title: Inhibition of rat corneal angiogenesis by a nuclease-resistant RNA aptamer specific for angiopoietin-2
  publication-title: Proc. Natl. Acad. Sci. USA
  contributor:
    fullname: Sullenger
– volume: 4
  start-page: 609
  year: 2004
  end-page: 618
  ident: bib42
  article-title: Photoaptamer arrays applied to multiplexed proteomic analysis
  publication-title: Proteomics
  contributor:
    fullname: Zichi
– volume: 16
  start-page: 165
  year: 2003
  end-page: 176
  ident: bib37
  article-title: Solid supports for microarray immunoassays
  publication-title: J. Mol. Recogn.
  contributor:
    fullname: Hoheisel
– start-page: 14
  year: 2002
  end-page: 23
  ident: bib1
  article-title: Antibody microarrays: promises and problems
  publication-title: Biotech. Suppl.
  contributor:
    fullname: Hoheisel
– volume: 273
  start-page: 14309
  year: 1998
  end-page: 14314
  ident: bib21
  article-title: RNA molecules that bind to and inhibit the active site of a tyrosine phosphatase
  publication-title: J. Biol. Chem.
  contributor:
    fullname: Ellington
– volume: 77
  start-page: 103
  year: 2002
  end-page: 112
  ident: bib7
  article-title: Protein array technology: the tool to bridge genomics and proteomics
  publication-title: Adv. Biochem. Eng. Biotechnol.
  contributor:
    fullname: Bussow
– volume: 3
  start-page: 283
  year: 1987
  end-page: 302
  ident: bib26
  article-title: Simple, rapid, quantitative, syncytium-forming microassay for the detection of human immunodeficiency virus neutralizing antibody
  publication-title: AIDS Res. Hum. Retroviruses
  contributor:
    fullname: Fischinger
– volume: 45
  start-page: 1628
  year: 1999
  end-page: 1650
  ident: bib12
  article-title: Aptamers: an emerging class of molecules that rival antibodies in diagnostics
  publication-title: Clin. Chem.
  contributor:
    fullname: Jayasena
– volume: 276
  start-page: 48644
  year: 2001
  end-page: 48654
  ident: bib17
  article-title: Tenascin-C aptamers are generated using tumor cells and purified protein
  publication-title: J. Biol. Chem.
  contributor:
    fullname: Warren
– volume: 343
  start-page: 17
  year: 2004
  end-page: 35
  ident: bib31
  article-title: Advances in recombinant antibody microarrays
  publication-title: Clin. Chim. Acta
  contributor:
    fullname: Hug
– volume: 112
  start-page: 101
  year: 1992
  end-page: 105
  ident: bib29
  article-title: Initiator oligonucleotides for the combination of chemical and enzymatic RNA synthesis
  publication-title: Gene
  contributor:
    fullname: Krupp
– volume: 7
  start-page: 55
  year: 2003
  end-page: 63
  ident: bib3
  article-title: Protein chip technology
  publication-title: Curr. Opin. Chem. Biol.
  contributor:
    fullname: Snyder
– volume: 13
  start-page: 14
  year: 2002
  end-page: 19
  ident: bib6
  article-title: Measuring proteins on microarrays
  publication-title: Curr. Opin. Biotechnol.
  contributor:
    fullname: Kingsmore
– volume: 372
  start-page: 44
  year: 2002
  end-page: 48
  ident: bib38
  article-title: Aptasensors—the future of biosensing?
  publication-title: Anal. Bioanal. Chem.
  contributor:
    fullname: O’Sullivan
– volume: 3
  year: 2002
  ident: bib44
  article-title: Beyond the proteome: non-coding regulatory RNAs
  publication-title: Genome Biol.
  contributor:
    fullname: Barciszewski
– volume: 1
  start-page: 13
  year: 2001
  end-page: 29
  ident: bib8
  article-title: Arrays for protein expression profiling: towards a viable alternative to two-dimensional gel electrophoresis?
  publication-title: Proteomics
  contributor:
    fullname: Pennington
– volume: 74
  start-page: 5
  year: 2000
  end-page: 13
  ident: bib10
  article-title: Aptamers as therapeutic and diagnostic agents
  publication-title: J. Biotechnol.
  contributor:
    fullname: Gold
– volume: 6
  start-page: 30
  year: 2002
  end-page: 34
  ident: bib19
  article-title: Optimizing aptamer activity for gene therapy applications using expression cassette SELEX
  publication-title: Mol. Ther.
  contributor:
    fullname: Sullenger
– volume: 75
  start-page: 4732
  year: 2003
  end-page: 4739
  ident: bib40
  article-title: Aptamer sensor arrays for the detection and quantification of proteins
  publication-title: Anal. Chem.
  contributor:
    fullname: Ellington
– volume: 2
  start-page: 28
  year: 1994
  end-page: 36
  ident: bib23
  article-title: Fitting a mixture model by expectation maximization to discover motifs in biopolymers
  publication-title: Proc. Int. Conf. Intell. Syst. Mol. Biol.
  contributor:
    fullname: Elkan
– volume: 61
  start-page: 7585
  year: 2001
  end-page: 7593
  ident: bib5
  article-title: Profiling of cancer cells using protein microarrays: discovery of novel radiation-regulated proteins
  publication-title: Cancer Res.
  contributor:
    fullname: Chinnaiyan
– volume: 99
  start-page: 7554
  year: 2002
  end-page: 7559
  ident: bib33
  article-title: Measuring absolute expression with microarrays with a calibrated reference sample and an extended signal intensity range
  publication-title: Proc. Natl. Acad. Sci. USA
  contributor:
    fullname: Church
– volume: 4
  start-page: 75
  year: 1998
  end-page: 89
  ident: bib16
  article-title: The limits of specificity: an experimental analysis with RNA aptamers to MS2 coat protein variants
  publication-title: Mol. Divers.
  contributor:
    fullname: Ellington
– volume: 21
  start-page: 1509
  year: 2003
  end-page: 1512
  ident: bib34
  article-title: Analyzing antibody specificity with whole proteome microarrays
  publication-title: Nat. Biotechnol.
  contributor:
    fullname: Schweitzer
– volume: 32
  start-page: 526
  year: 2002
  end-page: 532
  ident: bib2
  article-title: Protein microarrays and proteomics
  publication-title: Nat. Genet.
  contributor:
    fullname: MacBeath
– volume: 5
  start-page: 316
  year: 2004
  ident: 10.1016/j.ab.2004.11.027_bib43
  article-title: RNA regulation: a new genetics?
  publication-title: Nat. Rev. Genet
  doi: 10.1038/nrg1321
  contributor:
    fullname: Mattick
– volume: 74
  start-page: 15
  year: 2000
  ident: 10.1016/j.ab.2004.11.027_bib11
  article-title: In vitro selection of nucleic acids for diagnostic applications
  publication-title: J. Biotechnol.
  contributor:
    fullname: Hesselberth
– volume: 100
  start-page: 5028
  year: 2003
  ident: 10.1016/j.ab.2004.11.027_bib20
  article-title: Inhibition of rat corneal angiogenesis by a nuclease-resistant RNA aptamer specific for angiopoietin-2
  publication-title: Proc. Natl. Acad. Sci. USA
  doi: 10.1073/pnas.0831159100
  contributor:
    fullname: White
– volume: 112
  start-page: 101
  year: 1992
  ident: 10.1016/j.ab.2004.11.027_bib29
  article-title: Initiator oligonucleotides for the combination of chemical and enzymatic RNA synthesis
  publication-title: Gene
  doi: 10.1016/0378-1119(92)90309-D
  contributor:
    fullname: Pitulle
– volume: 6
  start-page: 30
  year: 2002
  ident: 10.1016/j.ab.2004.11.027_bib19
  article-title: Optimizing aptamer activity for gene therapy applications using expression cassette SELEX
  publication-title: Mol. Ther.
  doi: 10.1006/mthe.2002.0624
  contributor:
    fullname: Martell
– ident: 10.1016/j.ab.2004.11.027_bib27
– volume: 332
  start-page: 469
  year: 1988
  ident: 10.1016/j.ab.2004.11.027_bib25
  article-title: Quantitative infectivity assay for HIV-1 and-2
  publication-title: Nature
  doi: 10.1038/332469a0
  contributor:
    fullname: Nara
– volume: 8
  start-page: 250
  year: 2002
  ident: 10.1016/j.ab.2004.11.027_bib39
  article-title: High-throughput protein arrays: prospects for molecular diagnostics
  publication-title: Trends Mol. Med.
  doi: 10.1016/S1471-4914(02)02352-3
  contributor:
    fullname: Walter
– volume: 3
  start-page: 56
  year: 2003
  ident: 10.1016/j.ab.2004.11.027_bib4
  article-title: Antibody microarray profiling of human prostate cancer sera: antibody screening and identification of potential biomarkers
  publication-title: Proteomics
  doi: 10.1002/pmic.200390009
  contributor:
    fullname: Miller
– volume: 269
  start-page: 32051
  year: 1994
  ident: 10.1016/j.ab.2004.11.027_bib15
  article-title: Isozyme-specific inhibition of protein kinase C by RNA aptamers
  publication-title: J. Biol. Chem.
  doi: 10.1016/S0021-9258(18)31598-9
  contributor:
    fullname: Conrad
– volume: 77
  start-page: 103
  year: 2002
  ident: 10.1016/j.ab.2004.11.027_bib7
  article-title: Protein array technology: the tool to bridge genomics and proteomics
  publication-title: Adv. Biochem. Eng. Biotechnol.
  contributor:
    fullname: Eickhoff
– volume: 3
  start-page: 254
  year: 2003
  ident: 10.1016/j.ab.2004.11.027_bib41
  article-title: Antibody microarrays: an evaluation of production parameters
  publication-title: Proteomics
  doi: 10.1002/pmic.200390038
  contributor:
    fullname: Kusnezow
– volume: 75
  start-page: 4732
  year: 2003
  ident: 10.1016/j.ab.2004.11.027_bib40
  article-title: Aptamer sensor arrays for the detection and quantification of proteins
  publication-title: Anal. Chem.
  contributor:
    fullname: Kirby
– volume: 276
  start-page: 48644
  year: 2001
  ident: 10.1016/j.ab.2004.11.027_bib17
  article-title: Tenascin-C aptamers are generated using tumor cells and purified protein
  publication-title: J. Biol. Chem.
  doi: 10.1074/jbc.M104651200
  contributor:
    fullname: Hicke
– volume: 13
  start-page: 14
  year: 2002
  ident: 10.1016/j.ab.2004.11.027_bib6
  article-title: Measuring proteins on microarrays
  publication-title: Curr. Opin. Biotechnol.
  doi: 10.1016/S0958-1669(02)00278-1
  contributor:
    fullname: Schweitzer
– volume: 2
  start-page: 28
  year: 1994
  ident: 10.1016/j.ab.2004.11.027_bib23
  article-title: Fitting a mixture model by expectation maximization to discover motifs in biopolymers
  publication-title: Proc. Int. Conf. Intell. Syst. Mol. Biol.
  contributor:
    fullname: Bailey
– volume: 319
  start-page: 244
  year: 2003
  ident: 10.1016/j.ab.2004.11.027_bib28
  article-title: Aptamer-based biosensor arrays for detection and quantification of biological macromolecules
  publication-title: Anal. Biochem.
  doi: 10.1016/S0003-2697(03)00297-5
  contributor:
    fullname: McCauley
– volume: 32
  start-page: 526
  issue: Suppl
  year: 2002
  ident: 10.1016/j.ab.2004.11.027_bib2
  article-title: Protein microarrays and proteomics
  publication-title: Nat. Genet.
  doi: 10.1038/ng1037
  contributor:
    fullname: MacBeath
– volume: 3
  year: 2002
  ident: 10.1016/j.ab.2004.11.027_bib44
  article-title: Beyond the proteome: non-coding regulatory RNAs
  publication-title: Genome Biol.
  contributor:
    fullname: Szymanski
– volume: 45
  start-page: 1628
  year: 1999
  ident: 10.1016/j.ab.2004.11.027_bib12
  article-title: Aptamers: an emerging class of molecules that rival antibodies in diagnostics
  publication-title: Clin. Chem.
  doi: 10.1093/clinchem/45.9.1628
  contributor:
    fullname: Jayasena
– volume: 3
  start-page: 283
  year: 1987
  ident: 10.1016/j.ab.2004.11.027_bib26
  article-title: Simple, rapid, quantitative, syncytium-forming microassay for the detection of human immunodeficiency virus neutralizing antibody
  publication-title: AIDS Res. Hum. Retroviruses
  doi: 10.1089/aid.1987.3.283
  contributor:
    fullname: Nara
– volume: 372
  start-page: 44
  year: 2002
  ident: 10.1016/j.ab.2004.11.027_bib38
  article-title: Aptasensors—the future of biosensing?
  publication-title: Anal. Bioanal. Chem.
  doi: 10.1007/s00216-001-1189-3
  contributor:
    fullname: O’Sullivan
– volume: 61
  start-page: 7585
  year: 2001
  ident: 10.1016/j.ab.2004.11.027_bib5
  article-title: Profiling of cancer cells using protein microarrays: discovery of novel radiation-regulated proteins
  publication-title: Cancer Res.
  contributor:
    fullname: Sreekumar
– volume: 21
  start-page: 1509
  year: 2003
  ident: 10.1016/j.ab.2004.11.027_bib34
  article-title: Analyzing antibody specificity with whole proteome microarrays
  publication-title: Nat. Biotechnol.
  doi: 10.1038/nbt910
  contributor:
    fullname: Michaud
– volume: 343
  start-page: 17
  year: 2004
  ident: 10.1016/j.ab.2004.11.027_bib31
  article-title: Advances in recombinant antibody microarrays
  publication-title: Clin. Chim. Acta
  doi: 10.1016/j.cccn.2004.01.009
  contributor:
    fullname: Pavlickova
– volume: 270
  start-page: 103
  year: 1999
  ident: 10.1016/j.ab.2004.11.027_bib9
  article-title: Protein microarrays for gene expression and antibody screening
  publication-title: Anal. Biochem.
  doi: 10.1006/abio.1999.4063
  contributor:
    fullname: Lueking
– volume: 9
  start-page: 2525
  year: 2001
  ident: 10.1016/j.ab.2004.11.027_bib13
  article-title: Automated selection of anti-protein aptamers
  publication-title: Bioorg. Med. Chem.
  doi: 10.1016/S0968-0896(01)00028-1
  contributor:
    fullname: Cox
– volume: 24
  start-page: 1029
  year: 1996
  ident: 10.1016/j.ab.2004.11.027_bib18
  article-title: RNA aptamers that bind l-arginine with sub-micromolar dissociation constants and high enantioselectivity
  publication-title: Nucleic Acids Res.
  doi: 10.1093/nar/24.6.1029
  contributor:
    fullname: Geiger
– volume: 21
  start-page: 15
  year: 1999
  ident: 10.1016/j.ab.2004.11.027_bib32
  article-title: Making and reading microarrays
  publication-title: Nat. Genet
  doi: 10.1038/4439
  contributor:
    fullname: Cheung
– volume: 20
  start-page: 225
  year: 2002
  ident: 10.1016/j.ab.2004.11.027_bib35
  article-title: A perspective on protein microarrays
  publication-title: Nat. Biotechnol.
  doi: 10.1038/nbt0302-225
  contributor:
    fullname: Mitchell
– volume: 14
  start-page: 845
  year: 1998
  ident: 10.1016/j.ab.2004.11.027_bib14
  article-title: Automated RNA selection
  publication-title: Biotechnol. Prog.
  doi: 10.1021/bp980097h
  contributor:
    fullname: Cox
– volume: 74
  start-page: 5
  year: 2000
  ident: 10.1016/j.ab.2004.11.027_bib10
  article-title: Aptamers as therapeutic and diagnostic agents
  publication-title: J. Biotechnol.
  contributor:
    fullname: Brody
– volume: 99
  start-page: 7554
  year: 2002
  ident: 10.1016/j.ab.2004.11.027_bib33
  article-title: Measuring absolute expression with microarrays with a calibrated reference sample and an extended signal intensity range
  publication-title: Proc. Natl. Acad. Sci. USA
  doi: 10.1073/pnas.112683499
  contributor:
    fullname: Dudley
– start-page: 14
  year: 2002
  ident: 10.1016/j.ab.2004.11.027_bib1
  article-title: Antibody microarrays: promises and problems
  publication-title: Biotech. Suppl.
  doi: 10.2144/dec02kusnezow
  contributor:
    fullname: Kusnezow
– volume: 1
  start-page: 13
  year: 2001
  ident: 10.1016/j.ab.2004.11.027_bib8
  article-title: Arrays for protein expression profiling: towards a viable alternative to two-dimensional gel electrophoresis?
  publication-title: Proteomics
  doi: 10.1002/1615-9861(200101)1:1<13::AID-PROT13>3.0.CO;2-J
  contributor:
    fullname: Jenkins
– volume: 16
  start-page: 165
  year: 2003
  ident: 10.1016/j.ab.2004.11.027_bib37
  article-title: Solid supports for microarray immunoassays
  publication-title: J. Mol. Recogn.
  doi: 10.1002/jmr.625
  contributor:
    fullname: Kusnezow
– volume: 7
  start-page: 55
  year: 2003
  ident: 10.1016/j.ab.2004.11.027_bib3
  article-title: Protein chip technology
  publication-title: Curr. Opin. Chem. Biol.
  doi: 10.1016/S1367-5931(02)00005-4
  contributor:
    fullname: Zhu
– volume: 25
  start-page: 3389
  year: 1997
  ident: 10.1016/j.ab.2004.11.027_bib22
  article-title: Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
  publication-title: Nucleic Acids Res.
  doi: 10.1093/nar/25.17.3389
  contributor:
    fullname: Altschul
– volume: 4
  start-page: 75
  year: 1998
  ident: 10.1016/j.ab.2004.11.027_bib16
  article-title: The limits of specificity: an experimental analysis with RNA aptamers to MS2 coat protein variants
  publication-title: Mol. Divers.
  doi: 10.1023/A:1026401917416
  contributor:
    fullname: Hirao
– volume: 18
  start-page: 522
  year: 1965
  ident: 10.1016/j.ab.2004.11.027_bib24
  article-title: Continuous culture of human lymphoblasts from peripheral blood of a child with acute leukemia
  publication-title: Cancer
  doi: 10.1002/1097-0142(196504)18:4<522::AID-CNCR2820180418>3.0.CO;2-J
  contributor:
    fullname: Foley
– volume: 289
  start-page: 1760
  year: 2000
  ident: 10.1016/j.ab.2004.11.027_bib30
  article-title: Printing proteins as microarrays for high-throughput function determination
  publication-title: Science
  doi: 10.1126/science.289.5485.1760
  contributor:
    fullname: MacBeath
– volume: 29
  start-page: 389
  year: 2001
  ident: 10.1016/j.ab.2004.11.027_bib36
  article-title: The contributions of sex, genotype and age to transcriptional variance in Drosophila melanogaster
  publication-title: Nat. Genet.
  doi: 10.1038/ng766
  contributor:
    fullname: Jin
– volume: 273
  start-page: 14309
  year: 1998
  ident: 10.1016/j.ab.2004.11.027_bib21
  article-title: RNA molecules that bind to and inhibit the active site of a tyrosine phosphatase
  publication-title: J. Biol. Chem.
  doi: 10.1074/jbc.273.23.14309
  contributor:
    fullname: Bell
– volume: 32
  start-page: D95
  issue: Database issue
  year: 2004
  ident: 10.1016/j.ab.2004.11.027_bib45
  article-title: Aptamer database
  publication-title: Nucleic Acids Res.
  doi: 10.1093/nar/gkh094
  contributor:
    fullname: Lee
– volume: 4
  start-page: 609
  year: 2004
  ident: 10.1016/j.ab.2004.11.027_bib42
  article-title: Photoaptamer arrays applied to multiplexed proteomic analysis
  publication-title: Proteomics
  doi: 10.1002/pmic.200300631
  contributor:
    fullname: Bock
SSID ssj0011456
Score 2.1476417
Snippet High-throughput methods for generating aptamer microarrays are described. As a proof-of-principle, the microarrays were used to screen the affinity and...
SourceID proquest
crossref
pubmed
elsevier
SourceType Aggregation Database
Index Database
Publisher
StartPage 113
SubjectTerms Base Sequence
Biosensing Techniques
Biotinylation
Functional RNA
High-throughput screening
Microarray
Microarray Analysis - methods
Muramidase - immunology
Protein detection
Proteomics
RNA - chemistry
RNA aptamer
RNA-Binding Proteins - isolation & purification
Transcription, Genetic
Title Functional RNA microarrays for high-throughput screening of antiprotein aptamers
URI https://dx.doi.org/10.1016/j.ab.2004.11.027
https://www.ncbi.nlm.nih.gov/pubmed/15707941
https://search.proquest.com/docview/17800850
https://search.proquest.com/docview/67423890
Volume 338
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1LS8NAEF6KHvQi2vqoj7oHETykbUyyyR5DsVSLRYrF3sJkk0CFpqFND734253ZJIpgPXgqlA0s3wzz3vkYu4lMmYTCMw0FIjJQKSxDJoC6nNgqlvexA0B1yOeRGEzsp6kzrbFe9RaGxipL21_YdG2ty386JZqdbDajN75djD2kS2ooLf3QnFhnUafbH19jHhjuawZXOmzQ6bJVWcx4QagzxDbt8SRemd9d07bQU7ug_iE7KGNH7hfXO2K1OK2zhp9i3jzf8Fuupzl1mbzO9noVk1uDvfTReRU1Pz4e-XxOQ3iwXMJmxTFm5bSy2CgJe7J1ztGSYHaLPo0vEo7Iz_Qyh1nKIcuB6tzHbNJ_eO0NjJJJwVC27eRG6CkRuV1NSaMEIEpuHHmhjZm0E5ugZJLYmCgKRDJBPMA1wUQpOSDcLqDArBO2ky7S-IxxdP-KWnOKuHMdGWG8Y0VSRNKMPMsKVZPdVSAGWbEwI6gmyd4DCIn30sa8I0DAm8yqUA5-CD1Ae_7HV9eVQAIEkhockMaL9SowXU-v4Nt-QlBj2pN44rSQ5PcdHdoVaJvn_7rTBdvXO131cNol28mX6_gKo5U8bGl1bLFd_3E4GNHvcPw2_ATzXOlf
link.rule.ids 315,786,790,4516,24137,27946,27947,45609,45703
linkProvider Elsevier
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1LS8NAEF6KHupFtPVRX92DCB6iXZNsssdSLFXbIqLgbZlsEqjQtLTpoRd_uzObRBHUg9cwgeWbYV47Ox9j57FQaSRD4RiQsYNG4ToqBbTl1DOJukl8AOpDjsZy8OLdv_qvNdar3sLQWGXp-wufbr11-eW6RPN6PpnQG98O5h4qIDNULj0038RsoEOmffX-OeeB-b6lcCVph8TLu8piyAsiWyJe0SJPIpb5OTb9lnvaGNTfYdtl8si7xfl2WS3JGqzZzbBwnq75BbfjnLZP3mD1XkXl1mSPfYxeRdOPP427fEpTeLBYwHrJMWnltLPYKRl75qucoyvB8haDGp-lHKGf2G0Ok4zDPAdqdO-xl_7tc2_glFQKjvE8P3ei0Mg46FhOGiPBFzJI4jDysJT2EwFGpamHlaJEKFPEAwIBAtXkgww6gBpz99lGNsuSQ8Yx_hu6mzNEnuurGBMeN1YyViIOXTcyLXZZgajnxcYMXY2SvWmIiPjSw8JDI-At5lYo629a1-jQ__irXSlEI5B0wwFZMlsttQhCu4PvdwlJN9OhQomDQpNfZ_RpWaAnjv51pjarD55HQz28Gz8csy274NVOqp2wjXyxSk4xdcmjM2uaHx7o6VE
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Functional+RNA+microarrays+for+high-throughput+screening+of+antiprotein+aptamers&rft.jtitle=Analytical+biochemistry&rft.au=Collett%2C+J+R&rft.au=Cho%2C+E+J&rft.au=Lee%2C+J+F&rft.au=Levy%2C+M&rft.date=2005-03-01&rft.issn=0003-2697&rft.volume=338&rft.issue=1&rft.spage=113&rft.epage=123&rft_id=info:doi/10.1016%2Fj.ab.2004.11.027&rft.externalDBID=NO_FULL_TEXT
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0003-2697&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0003-2697&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0003-2697&client=summon