Force properties of skinned cardiac muscle following increasing volumes of aerobic exercise in rats

• Published in: Journal of applied physiology (1985) Vol. 125; no. 2; pp. 495 - 503
• Main Authors: Boldt, Kevin R, Rios, Jaqueline L, Joumaa, Venus, Herzog, Walter
• Format: Journal Article
• Language: English
• Published: United States American Physiological Society 01.08.2018
• Subjects: Adaptation; Animals; Cardiac muscle; Cardiomyocytes; Exercise; Exercise physiology; Fitness; Heart; Mechanical properties; Mechanical tests; Muscle recovery; Muscles; Myocardium; Physical fitness; Physical training; Skin; Treadmills; excessive exercise; treadmill exercise; heart
• ISSN: 8750-7587; 1522-1601
• DOI: 10.1152/japplphysiol.00631.2017

The positive effects of chronic endurance exercise training on health and performance have been well documented. These positive effects have been evaluated primarily at the structural level, and work has begun to evaluate mechanical adaptations of the myocardium. However, it remains poorly understood how the volume of exercise training affects cardiac adaptation. To gain some understanding, we subjected 3-mo-old Sprague-Dawley rats ( n = 23) to treadmill running for 11 wk at one of three exercise volumes (moderate, high, and extra high). Following training, hearts were excised and mechanical testing was completed on skinned trabecular fiber bundles. Performance on a maximal fitness test was dose dependent on training volume, where greater levels of training led to greater performance. No differences were observed between animals from any group for maximal active stress and passive stress at a sarcomere length of 2.2 µm. Heart mass and passive stress at sarcomere lengths beyond 2.4 µm increased in a dose-dependent manner for animals in the control and moderate- and high-duration groups. However, hearts from animals in the extra high-duration group presented with inhibited responses for heart mass and passive stress, despite performing greatest on a graded treadmill fitness test. These results suggest that heart mass and passive stress adapt in a dose-dependent manner, until exercise becomes excessive and adaptation is inhibited. Our findings are in agreement with the beneficial role exercise has in cardiac adaptation. However, excessive exercise comes with risks of maladaptation, which must be weighed against the desire to increase performance. NEW & NOTEWORTHY For the first time, we present findings on cardiac trabecular muscle passive stiffness and show the effect of excessive exercise on the heart. We demonstrated that heart mass increases with exercise until a maximum, after which greater exercise volume results in inhibited adaptation. At paraphysiological lengths, passive stiffness increases with exercise but to a lesser degree with excessive training. Despite greater performance on graded exercise tests, animals in the highest trained group exhibited possible maladaptation.