Design and evaluation of biological gate circuits and their therapeutic applications in a model of multidrug resistant cancers

• Published in: Biotechnology letters Vol. 42; no. 8; pp. 1419 - 1429
• Main Authors: Soheili, Naeimeh, Eshghi, Mohammad, Emadi-Baygi, Modjtaba, Mirzaei, Seyed Abbas, Heidari, Razieh, Hosseinzadeh, Mehdi
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.08.2020; Springer Nature B.V
• Subjects: Antibiotics; Antineoplastic Agents - pharmacology; Applied Microbiology; Biochemistry; Biomedical and Life Sciences; Biotechnology; Butyric Acid - pharmacology; Cancer; Cancer therapies; cancer therapy; Circuit design; Circuits; Doxycycline; Doxycycline - pharmacology; Drug resistance; Drug Resistance, Multiple - genetics; Drug Resistance, Neoplasm - genetics; Gates (circuits); Gene expression; Gene Expression Regulation, Neoplastic - drug effects; Gene Expression Regulation, Neoplastic - genetics; Genetic Vectors - genetics; Humans; Lentivirus - genetics; Life Sciences; MCF-7 Cells; MDR1 protein; Microbiology; Models, Biological; Multidrug resistance; Multidrug resistant organisms; multiple drug resistance; Original Research Paper; P-Glycoprotein; RNA, Small Interfering - genetics; RNA, Small Interfering - metabolism; Sodium; Sodium butyrate; synthetic biology; Synthetic Biology - methods; synthetic genes; Therapeutic applications; Transcription; transcription (genetics); Synthetic biology; Biological gate; NOT-IF gate; shRNA; Cancer therapy; Multidrug resistance

Objectives Synthetic biology is primarily an emerging research field that consists of designing new synthetic gene circuits dedicated to targeted functions and therapies such as cancer therapy. In this study, a genetic logic NOT-IF gate is used to reduce the multidrug resistance and facilitate the m...