Single Image-Based Vignetting Correction for Improving the Consistency of Neural Activity Analysis in 2-Photon Functional Microscopy

• Published in: Frontiers in neuroinformatics Vol. 15; p. 674439
• Main Authors: Li, Dong, Wang, Guangyu, Werner, René, Xie, Hong, Guan, Ji-Song, Hilgetag, Claus C.
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Research Foundation 05.01.2022; Frontiers Media S.A
• Subjects: Activity patterns; functional microscopic imaging; image analysis; Image processing; imaging artifacts; Microscopy; Nervous system; neural activity; Neuroinformatics; Pipelines; Protein expression; vignetting correction; vignetting correction; imaging artifacts; neural activity; image analysis; functional microscopic imaging

High-resolution functional 2-photon microscopy of neural activity is a cornerstone technique in current neuroscience, enabling, for instance, the image-based analysis of relations of the organization of local neuron populations and their temporal neural activity patterns. Interpreting local image in...