Highly efficient genome editing in N. gerenzanensis using an inducible CRISPR/Cas9–RecA system

• Published in: Biotechnology letters Vol. 42; no. 9; pp. 1699 - 1706
• Main Authors: Yue, Xue, Xia, Tianyu, Wang, Shuai, Dong, Huijun, Li, Yongquan
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.09.2020; Springer Nature B.V
• Subjects: Actinobacteria - genetics; Actinomyces; Actinomyces - genetics; Applied Microbiology; Bacterial Proteins - genetics; Biochemistry; Biomedical and Life Sciences; Biotechnology; CRISPR; CRISPR-Cas systems; CRISPR-Cas Systems - genetics; Editing; Escherichia coli - genetics; Gene Editing - methods; Genome editing; Genomes; Homologous recombination; Homology; Life Sciences; Microbiology; mutants; Mutation - genetics; Nonomuraea; Original Research Paper; Rec A Recombinases - genetics; RecA protein; Recombinase; Genome editing; CRISPR/Cas9; A40926; RecA; N. gerenzanensis

Objective To develop an inducible CRISPR/Cas9–Recombinase A (RecA) system to manipulate genes in Nonomuraea gerenzanensis effectively. Results Compared with traditional homologous recombination, the inducible CRISPR/Cas9 system achieved 68.8% editing efficiency, whereas, with both the inducible Cas9...