Topological studies of cytochromes P-450scc and P-45011 beta in bovine adrenocortical inner mitochondrial membranes. Effects of controlled tryptic digestion

• Published in: The Journal of biological chemistry Vol. 254; no. 20; pp. 10443 - 10448
• Main Authors: Churchill, P F, Kimura, T
• Format: Journal Article
• Language: English
• Published: United States American Society for Biochemistry and Molecular Biology 25.10.1979
• Subjects: Adrenal Cortex - ultrastructure; Animals; Cattle; Cytochrome P-450 Enzyme System - analysis; Dithionite; Hydroxycholesterols - pharmacology; Intracellular Membranes - drug effects; Intracellular Membranes - ultrastructure; Mitochondria - drug effects; Mitochondria - ultrastructure; Pregnenolone - pharmacology; Spectrophotometry; Trypsin

The topology of the steroid hydroxylase complexes in bovine adrenocortical mitochondria were studied by using controlled digestion with trypsin of purified inner mitochondrial membranes. Inhibition of steroid hydroxylase activity by trypsin was only observed in inner mitochondrial membranes which ha...