Protection from COVID-19 with a VSV-based vaccine expressing the spike and nucleocapsid proteins

Successful vaccine efforts countering the COVID-19 pandemic are centralized around the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) protein as viral antigen and have greatly reduced the morbidity and mortality associated with COVID-19. Since the start of this pandemic, SARS...

Full description

Saved in:
Bibliographic Details
Published inFrontiers in immunology Vol. 13; p. 1025500
Main Authors O’Donnell, Kyle L., Gourdine, Tylisha, Fletcher, Paige, Clancy, Chad S., Marzi, Andrea
Format Journal Article
LanguageEnglish
Published Frontiers Media S.A 24.10.2022
Subjects
hamster model
Immunology
intranasal vaccination
SARS-CoV-2
severe acute respiratory syndrome coronavirus 2
vesicular stomatitis virus
Online AccessGet full text

Cover

Abstract Successful vaccine efforts countering the COVID-19 pandemic are centralized around the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) protein as viral antigen and have greatly reduced the morbidity and mortality associated with COVID-19. Since the start of this pandemic, SARS-CoV-2 has evolved resulting in new variants of concern (VOC) challenging the vaccine-established immunologic memory. We show that vaccination with a vesicular stomatitis virus (VSV)-based vaccine expressing the SARS-CoV-2 S plus the conserved nucleocapsid (N) protein was protective in a hamster challenge model when a single dose was administered 28 or 10 days prior to challenge, respectively. In this study, only intranasal vaccination resulted in protection against challenge with multiple VOC highlighting that the addition of the N protein indeed improved protective efficacy. This data demonstrates the ability of a VSV-based dual-antigen vaccine to reduce viral shedding and protect from disease caused by SARS-CoV-2 VOC.
AbstractList Successful vaccine efforts countering the COVID-19 pandemic are centralized around the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) protein as viral antigen and have greatly reduced the morbidity and mortality associated with COVID-19. Since the start of this pandemic, SARS-CoV-2 has evolved resulting in new variants of concern (VOC) challenging the vaccine-established immunologic memory. We show that vaccination with a vesicular stomatitis virus (VSV)-based vaccine expressing the SARS-CoV-2 S plus the conserved nucleocapsid (N) protein was protective in a hamster challenge model when a single dose was administered 28 or 10 days prior to challenge, respectively. In this study, only intranasal vaccination resulted in protection against challenge with multiple VOC highlighting that the addition of the N protein indeed improved protective efficacy. This data demonstrates the ability of a VSV-based dual-antigen vaccine to reduce viral shedding and protect from disease caused by SARS-CoV-2 VOC.
Author Fletcher, Paige
Clancy, Chad S.
O’Donnell, Kyle L.
Marzi, Andrea
Gourdine, Tylisha
AuthorAffiliation 1 Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Hamilton, MT , United States
2 Rocky Mountain Veterinary Branch, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Hamilton, MT , United States
AuthorAffiliation_xml – name: 2 Rocky Mountain Veterinary Branch, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Hamilton, MT , United States
– name: 1 Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Hamilton, MT , United States
Author_xml – sequence: 1
  givenname: Kyle L.
  surname: O’Donnell
  fullname: O’Donnell, Kyle L.
– sequence: 2
  givenname: Tylisha
  surname: Gourdine
  fullname: Gourdine, Tylisha
– sequence: 3
  givenname: Paige
  surname: Fletcher
  fullname: Fletcher, Paige
– sequence: 4
  givenname: Chad S.
  surname: Clancy
  fullname: Clancy, Chad S.
– sequence: 5
  givenname: Andrea
  surname: Marzi
  fullname: Marzi, Andrea
BookMark eNpVkctu1DAUhi1UJMrQF2DlJZsMvsfeIKHhNlKlIgGzNY5zMuOS2MFOSvv2ZDojRM_mXPX9R_pfoouYIiD0mpI159q87cIwzGtGGFtTwqQk5Bm6pEqJijMmLv6rX6CrUm7JEsJwzuUl-vk1pwn8FFLEXU4D3tzsth8qavCfMB2ww7tvu6pxBVp857wPETDcjxlKCXGPpwPgMoZfgF1scZx9D8m7sYQWj0duiOUVet65vsDVOa_Qj08fv2--VNc3n7eb99eVF0JOlYTGq7o1FJyDrnFSeC5A-rozElznayMaXjeKGM1rqlqpma-9k74hVFHi-QptT9w2uVs75jC4_GCTC_ZxkPLeujyF5UMrjDKcLTpaECFAa2p4x0jrQZvWL80KvTuxxrkZYJnHKbv-CfTpJoaD3ac7axTXVB4Bb86AnH7PUCY7hOKh712ENBfLai6pqhnVyyk7nfqcSsnQ_ZOhxB7ttY_22qO99mwv_wucFpxt
CitedBy_id crossref_primary_10_1038_s41541_024_00814_2
crossref_primary_10_1128_spectrum_00503_23
crossref_primary_10_3389_fimmu_2023_1188605
crossref_primary_10_15212_ZOONOSES_2023_0003
crossref_primary_10_3390_vaccines11121810
crossref_primary_10_1038_s41598_023_48397_7
crossref_primary_10_1155_2024_9313267
Cites_doi 10.1038/s41598-018-29401-x
10.3201/eid2506.181620
10.3390/vaccines10030435
10.1016/s0198-8859(99)00155-x
10.1056/NEJMoa2024671
10.1016/j.immuni.2020.10.006
10.1002/cti2.1219
10.4049/jimmunol.2100421
10.1126/science.1086418
10.1016/j.cell.2020.06.043
10.4172/2157-2526.S1-004
10.1016/j.xcrm.2020.100121
10.1016/j.ebiom.2022.104203
10.1139/W08-139
10.3389/fphar.2020.579330
10.1126/science.1085953
10.1056/NEJMoa2034201
10.1128/JVI.02002-20
10.1038/s41586-020-2798-3
10.1038/s41579-021-00573-0
10.1093/infdis/jir379
10.1038/s41385-021-00461-z
10.1038/s41591-021-01318-5
10.1016/S0140-6736(03)14962-8
10.1101/2020.07.31.20165647
10.1086/422040
10.1038/s41591-020-01143-2
10.1038/mi.2015.127
10.1016/j.cell.2020.08.017
10.1128/mbio.03379-21
10.1056/NEJMoa2035389
10.1016/j.immuni.2021.04.006
10.1007/s11262-004-4586-9
10.1016/j.antiviral.2006.05.010
10.1016/j.isci.2021.102096
10.1016/j.ebiom.2022.104138
10.1172/JCI145476
10.3389/fimmu.2021.788235
10.1016/S0140-6736(03)13412-5
10.1016/j.cell.2020.08.026
10.1128/JVI.01925-19
10.1016/j.chom.2020.03.002
10.1016/j.ijbiomac.2022.03.058
10.1371/journal.pntd.0003736
10.1002/path.1570
10.1016/j.ebiom.2021.103675
10.1038/s41590-020-0782-6
10.1038/s41586-020-2608-y
10.1016/j.vaccine.2005.01.036
ContentType Journal Article
Copyright Copyright © 2022 O’Donnell, Gourdine, Fletcher, Clancy and Marzi 2022 O’Donnell, Gourdine, Fletcher, Clancy and Marzi
Copyright_xml – notice: Copyright © 2022 O’Donnell, Gourdine, Fletcher, Clancy and Marzi 2022 O’Donnell, Gourdine, Fletcher, Clancy and Marzi
DBID AAYXX
CITATION
7X8
5PM
DOA
DOI 10.3389/fimmu.2022.1025500
DatabaseName CrossRef
MEDLINE - Academic
PubMed Central (Full Participant titles)
DOAJ Directory of Open Access Journals
DatabaseTitle CrossRef
MEDLINE - Academic
DatabaseTitleList CrossRef
Database_xml – sequence: 1
  dbid: DOA
  name: Directory of Open Access Journals
  url: https://www.doaj.org/
  sourceTypes: Open Website
DeliveryMethod fulltext_linktorsrc
Discipline Biology
EISSN 1664-3224
EndPage 1025500
ExternalDocumentID oai_doaj_org_article_496932aae84044e88193f20dce89dc19
10_3389_fimmu_2022_1025500
GrantInformation_xml – fundername: ;
GroupedDBID 53G
5VS
9T4
AAFWJ
AAKDD
AAYXX
ACGFO
ACGFS
ACXDI
ADBBV
ADRAZ
AENEX
AFPKN
ALMA_UNASSIGNED_HOLDINGS
AOIJS
BAWUL
BCNDV
CITATION
DIK
EBS
EMOBN
GROUPED_DOAJ
GX1
HYE
IAO
IEA
IHR
IHW
KQ8
M48
M~E
OK1
PGMZT
RNS
RPM
7X8
5PM
ID FETCH-LOGICAL-c445t-5ebc67d91eaaefba54c34e5c7f95eafc794b37b60983716d582c7ca5cb01610c3
IEDL.DBID RPM
ISSN 1664-3224
IngestDate Tue Oct 22 15:14:56 EDT 2024
Tue Sep 17 21:30:44 EDT 2024
Sat Oct 05 05:57:04 EDT 2024
Thu Nov 21 21:22:41 EST 2024
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Language English
License This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c445t-5ebc67d91eaaefba54c34e5c7f95eafc794b37b60983716d582c7ca5cb01610c3
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Edited by: Arzu Didem Yalcin, Academia Sinica, Taiwan
This article was submitted to Vaccines and Molecular Therapeutics, a section of the journal Frontiers in Immunology
Reviewed by: Diego Cantoni, MRC-University of Glasgow Centre For Virus Research, United Kingdom; Vidya Avinash Arankalle, National Institute of Virology (ICMR), India
OpenAccessLink https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9638159/
PQID 2735167218
PQPubID 23479
PageCount 1
ParticipantIDs doaj_primary_oai_doaj_org_article_496932aae84044e88193f20dce89dc19
pubmedcentral_primary_oai_pubmedcentral_nih_gov_9638159
proquest_miscellaneous_2735167218
crossref_primary_10_3389_fimmu_2022_1025500
PublicationCentury 2000
PublicationDate 2022-10-24
PublicationDateYYYYMMDD 2022-10-24
PublicationDate_xml – month: 10
  year: 2022
  text: 2022-10-24
  day: 24
PublicationDecade 2020
PublicationTitle Frontiers in immunology
PublicationYear 2022
Publisher Frontiers Media S.A
Publisher_xml – name: Frontiers Media S.A
References Ellis (B38) 2000; 61
O’Donnell (B21) 2022; 12
Harvey (B5) 2021; 19
Lee (B49) 2020; 1
Zhu (B10) 2005; 30
Lineburg (B15) 2021; 54
van Doremalen (B26) 2020; 586
Marzi (B18) 2011
Tylor (B30) 2009; 55
Cong (B11) 2020; 94
Tsuda (B22) 2011
Zheng (B45) 2022; 15
Planas (B28) 2021; 27
Sadoff (B4) 2021; 384
Safronetz (B19) 2015; 9
B1
Kared (B33) 2021; 131
Hamming (B48) 2004; 203
Emanuel (B23) 2018; 8
Robbins (B46) 2022; 82
Matchett (B13) 2021; 207
Marra (B7) 2003; 300
Schulien (B35) 2021; 27
Corbett (B2) 2020; 383
Holmes (B9) 2003; 300
Peiris (B44) 2003; 361
Espeseth (B47) 2022
Tan (B31) 2006; 72
Okada (B16) 2005; 23
Souza (B6) 2022; 208
Sekine (B36) 2020; 183
Leung (B12) 2004; 190
Suresh (B50) 2020; 11
Baden (B41) 2021; 384
Gao (B14) 2003; 362
Peng (B34) 2020; 21
Liu (B43) 2016; 9
Hassan (B3) 2020; 183
Lee (B17) 2021; 95
Snyder (B37) 2020
Szeto (B40) 2021; 24
Mire (B20) 2019; 25
Krammer (B42) 2020; 586
Furuyama (B24) 2022
O'Donnell (B27) 2022; 10
O'Donnell (B25) 2021; 73
Lineburg (B39) 2020; 9
Ferretti (B32) 2020; 53
Korber (B29) 2020; 182
Grifoni (B8) 2020; 27
References_xml – volume: 8
  start-page: 11043
  year: 2018
  ident: B23
  article-title: A VSV-based zika virus vaccine protects mice from lethal challenge
  publication-title: Sci Rep
  doi: 10.1038/s41598-018-29401-x
  contributor:
    fullname: Emanuel
– volume: 25
  year: 2019
  ident: B20
  article-title: Use of single-injection recombinant vesicular stomatitis virus vaccine to protect nonhuman primates against lethal nipah virus disease
  publication-title: Emerg Infect Dis
  doi: 10.3201/eid2506.181620
  contributor:
    fullname: Mire
– volume: 10
  year: 2022
  ident: B27
  article-title: VSV-based vaccines reduce virus shedding and viral load in hamsters infected with SARS-CoV-2 variants of concern
  publication-title: Vaccines (Basel)
  doi: 10.3390/vaccines10030435
  contributor:
    fullname: O'Donnell
– volume: 61
  year: 2000
  ident: B38
  article-title: Frequencies of HLA-A2 alleles in five U.S. population groups. predominance of A*02011 and identification of HLA-A*0231
  publication-title: Hum Immunol
  doi: 10.1016/s0198-8859(99)00155-x
  contributor:
    fullname: Ellis
– volume: 383
  year: 2020
  ident: B2
  article-title: Evaluation of the mRNA-1273 vaccine against SARS-CoV-2 in nonhuman primates
  publication-title: N Engl J Med
  doi: 10.1056/NEJMoa2024671
  contributor:
    fullname: Corbett
– volume: 53
  start-page: 1095
  year: 2020
  ident: B32
  article-title: Unbiased screens show CD8(+) T cells of COVID-19 patients recognize shared epitopes in SARS-CoV-2 that largely reside outside the spike protein
  publication-title: Immunity.
  doi: 10.1016/j.immuni.2020.10.006
  contributor:
    fullname: Ferretti
– volume: 9
  year: 2020
  ident: B39
  article-title: Rapid detection of SARS-CoV-2-specific memory T-cell immunity in recovered COVID-19 cases
  publication-title: Clin Transl Immunol
  doi: 10.1002/cti2.1219
  contributor:
    fullname: Lineburg
– volume: 207
  year: 2021
  ident: B13
  article-title: Cutting edge: Nucleocapsid vaccine elicits spike-independent SARS-CoV-2 protective immunity
  publication-title: J Immunol
  doi: 10.4049/jimmunol.2100421
  contributor:
    fullname: Matchett
– volume: 300
  year: 2003
  ident: B9
  article-title: Virology. the SARS coronavirus: a postgenomic era
  publication-title: Science.
  doi: 10.1126/science.1086418
  contributor:
    fullname: Holmes
– volume: 182
  start-page: 812
  year: 2020
  ident: B29
  article-title: Tracking changes in SARS-CoV-2 spike: Evidence that D614G increases infectivity of the COVID-19 virus
  publication-title: Cell.
  doi: 10.1016/j.cell.2020.06.043
  contributor:
    fullname: Korber
– year: 2011
  ident: B18
  article-title: Vesicular stomatitis virus-based vaccines for prophylaxis and treatment of filovirus infections
  publication-title: J Bioterror Biodef
  doi: 10.4172/2157-2526.S1-004
  contributor:
    fullname: Marzi
– volume: 1
  start-page: 100121
  year: 2020
  ident: B49
  article-title: Oral SARS-CoV-2 inoculation establishes subclinical respiratory infection with virus shedding in golden Syrian hamsters
  publication-title: Cell Rep Med
  doi: 10.1016/j.xcrm.2020.100121
  contributor:
    fullname: Lee
– start-page: 104203
  year: 2022
  ident: B47
  article-title: Preclinical immunogenicity and efficacy of a candidate COVID-19 vaccine based on a vesicular stomatitis virus-SARS-CoV-2 chimera
  publication-title: EBioMedicine
  doi: 10.1016/j.ebiom.2022.104203
  contributor:
    fullname: Espeseth
– volume: 55
  year: 2009
  ident: B30
  article-title: The SR-rich motif in SARS-CoV nucleocapsid protein is important for virus replication
  publication-title: Can J Microbiol
  doi: 10.1139/W08-139
  contributor:
    fullname: Tylor
– volume: 11
  year: 2020
  ident: B50
  article-title: Tissue distribution of ACE2 protein in Syrian golden hamster (Mesocricetus auratus) and its possible implications in SARS-CoV-2 related studies
  publication-title: Front Pharmacol
  doi: 10.3389/fphar.2020.579330
  contributor:
    fullname: Suresh
– volume: 300
  year: 2003
  ident: B7
  article-title: The genome sequence of the SARS-associated coronavirus
  publication-title: Science.
  doi: 10.1126/science.1085953
  contributor:
    fullname: Marra
– volume: 384
  year: 2021
  ident: B4
  article-title: Interim results of a phase 1-2a trial of Ad26.COV2.S covid-19 vaccine
  publication-title: N Engl J Med
  doi: 10.1056/NEJMoa2034201
  contributor:
    fullname: Sadoff
– volume: 95
  year: 2021
  ident: B17
  article-title: Identification of SARS-CoV-2 nucleocapsid and spike T-cell epitopes for assessing T-cell immunity
  publication-title: J Virol
  doi: 10.1128/JVI.02002-20
  contributor:
    fullname: Lee
– volume: 586
  year: 2020
  ident: B42
  article-title: SARS-CoV-2 vaccines in development
  publication-title: Nature.
  doi: 10.1038/s41586-020-2798-3
  contributor:
    fullname: Krammer
– volume: 19
  year: 2021
  ident: B5
  article-title: SARS-CoV-2 variants, spike mutations and immune escape
  publication-title: Nat Rev Microbiol
  doi: 10.1038/s41579-021-00573-0
  contributor:
    fullname: Harvey
– year: 2011
  ident: B22
  article-title: Protective efficacy of a bivalent recombinant vesicular stomatitis virus vaccine in the Syrian hamster model of lethal Ebola virus infection
  publication-title: J Infect Dis
  doi: 10.1093/infdis/jir379
  contributor:
    fullname: Tsuda
– volume: 15
  year: 2022
  ident: B45
  article-title: Tissue resident memory T cells in the respiratory tract
  publication-title: Mucosal Immunol
  doi: 10.1038/s41385-021-00461-z
  contributor:
    fullname: Zheng
– volume: 27
  year: 2021
  ident: B28
  article-title: Sensitivity of infectious SARS-CoV-2 B.1.1.7 and B.1.351 variants to neutralizing antibodies
  publication-title: Nat Med
  doi: 10.1038/s41591-021-01318-5
  contributor:
    fullname: Planas
– volume: 362
  year: 2003
  ident: B14
  article-title: Effects of a SARS-associated coronavirus vaccine in monkeys
  publication-title: Lancet.
  doi: 10.1016/S0140-6736(03)14962-8
  contributor:
    fullname: Gao
– year: 2020
  ident: B37
  article-title: Magnitude and dynamics of the T-cell response to SARS-CoV-2 infection at both individual and population levels
  publication-title: medRxiv
  doi: 10.1101/2020.07.31.20165647
  contributor:
    fullname: Snyder
– volume: 190
  year: 2004
  ident: B12
  article-title: Antibody response of patients with severe acute respiratory syndrome (SARS) targets the viral nucleocapsid
  publication-title: J Infect Dis
  doi: 10.1086/422040
  contributor:
    fullname: Leung
– volume: 27
  start-page: 78
  year: 2021
  ident: B35
  article-title: Characterization of pre-existing and induced SARS-CoV-2-specific CD8(+) T cells
  publication-title: Nat Med
  doi: 10.1038/s41591-020-01143-2
  contributor:
    fullname: Schulien
– volume: 9
  year: 2016
  ident: B43
  article-title: Spatiotemporal interplay of severe acute respiratory syndrome coronavirus and respiratory mucosal cells drives viral dissemination in rhesus macaques
  publication-title: Mucosal Immunol
  doi: 10.1038/mi.2015.127
  contributor:
    fullname: Liu
– volume: 183
  start-page: 158
  year: 2020
  ident: B36
  article-title: Robust T cell immunity in convalescent individuals with asymptomatic or mild COVID-19
  publication-title: Cell.
  doi: 10.1016/j.cell.2020.08.017
  contributor:
    fullname: Sekine
– ident: B1
– year: 2022
  ident: B24
  article-title: Rapid protection from COVID-19 in nonhuman primates vaccinated intramuscularly but not intranasally with a single dose of a vesicular stomatitis virus-based vaccine
  publication-title: mBio
  doi: 10.1128/mbio.03379-21
  contributor:
    fullname: Furuyama
– volume: 384
  year: 2021
  ident: B41
  article-title: Efficacy and safety of the mRNA-1273 SARS-CoV-2 vaccine
  publication-title: N Engl J Med
  doi: 10.1056/NEJMoa2035389
  contributor:
    fullname: Baden
– volume: 54
  start-page: 1055
  year: 2021
  ident: B15
  article-title: CD8(+) T cells specific for an immunodominant SARS-CoV-2 nucleocapsid epitope cross-react with selective seasonal coronaviruses
  publication-title: Immunity.
  doi: 10.1016/j.immuni.2021.04.006
  contributor:
    fullname: Lineburg
– volume: 30
  start-page: 93
  year: 2005
  ident: B10
  article-title: Isolation of virus from a SARS patient and genome-wide analysis of genetic mutations related to pathogenesis and epidemiology from 47 SARS-CoV isolates
  publication-title: Virus Genes
  doi: 10.1007/s11262-004-4586-9
  contributor:
    fullname: Zhu
– volume: 72
  start-page: 78
  year: 2006
  ident: B31
  article-title: Understanding the accessory viral proteins unique to the severe acute respiratory syndrome (SARS) coronavirus
  publication-title: Antiviral Res
  doi: 10.1016/j.antiviral.2006.05.010
  contributor:
    fullname: Tan
– volume: 24
  start-page: 102096
  year: 2021
  ident: B40
  article-title: The presentation of SARS-CoV-2 peptides by the common HLA-a( *)02:01 molecule
  publication-title: iScience.
  doi: 10.1016/j.isci.2021.102096
  contributor:
    fullname: Szeto
– volume: 82
  start-page: 104138
  year: 2022
  ident: B46
  article-title: Safety and immunogenicity of intramuscular, single-dose V590 (rVSV-SARS-CoV-2 vaccine) in healthy adults: Results from a phase 1 randomised, double-blind, placebo-controlled, dose-ranging trial
  publication-title: EBioMedicine.
  doi: 10.1016/j.ebiom.2022.104138
  contributor:
    fullname: Robbins
– volume: 131
  year: 2021
  ident: B33
  article-title: SARS-CoV-2-specific CD8+ T cell responses in convalescent COVID-19 individuals
  publication-title: J Clin Invest
  doi: 10.1172/JCI145476
  contributor:
    fullname: Kared
– volume: 12
  year: 2022
  ident: B21
  article-title: Optimization of single-dose VSV-based COVID-19 vaccination in hamsters
  publication-title: Front Immunol
  doi: 10.3389/fimmu.2021.788235
  contributor:
    fullname: O’Donnell
– volume: 361
  year: 2003
  ident: B44
  article-title: Clinical progression and viral load in a community outbreak of coronavirus-associated SARS pneumonia: a prospective study
  publication-title: Lancet.
  doi: 10.1016/S0140-6736(03)13412-5
  contributor:
    fullname: Peiris
– volume: 183
  start-page: 169
  year: 2020
  ident: B3
  article-title: A single-dose intranasal ChAd vaccine protects upper and lower respiratory tracts against SARS-CoV-2
  publication-title: Cell.
  doi: 10.1016/j.cell.2020.08.026
  contributor:
    fullname: Hassan
– volume: 94
  year: 2020
  ident: B11
  article-title: Nucleocapsid protein recruitment to replication-transcription complexes plays a crucial role in coronaviral life cycle
  publication-title: J Virol
  doi: 10.1128/JVI.01925-19
  contributor:
    fullname: Cong
– volume: 27
  start-page: 671
  year: 2020
  ident: B8
  article-title: A sequence homology and bioinformatic approach can predict candidate targets for immune responses to SARS-CoV-2
  publication-title: Cell Host Microbe
  doi: 10.1016/j.chom.2020.03.002
  contributor:
    fullname: Grifoni
– volume: 208
  year: 2022
  ident: B6
  article-title: The spike glycoprotein of SARS-CoV-2: A review of how mutations of spike glycoproteins have driven the emergence of variants with high transmissibility and immune escape
  publication-title: Int J Biol Macromol
  doi: 10.1016/j.ijbiomac.2022.03.058
  contributor:
    fullname: Souza
– volume: 9
  year: 2015
  ident: B19
  article-title: A recombinant vesicular stomatitis virus-based lassa fever vaccine protects guinea pigs and macaques against challenge with geographically and genetically distinct lassa viruses
  publication-title: Plos Negl Trop Dis
  doi: 10.1371/journal.pntd.0003736
  contributor:
    fullname: Safronetz
– volume: 203
  year: 2004
  ident: B48
  article-title: Tissue distribution of ACE2 protein, the functional receptor for SARS coronavirus. a first step in understanding SARS pathogenesis
  publication-title: J Pathol
  doi: 10.1002/path.1570
  contributor:
    fullname: Hamming
– volume: 73
  start-page: 103675
  year: 2021
  ident: B25
  article-title: Pathogenic and transcriptomic differences of emerging SARS-CoV-2 variants in the Syrian golden hamster model
  publication-title: EBioMedicine.
  doi: 10.1016/j.ebiom.2021.103675
  contributor:
    fullname: O'Donnell
– volume: 21
  year: 2020
  ident: B34
  article-title: Broad and strong memory CD4(+) and CD8(+) T cells induced by SARS-CoV-2 in UK convalescent individuals following COVID-19
  publication-title: Nat Immunol
  doi: 10.1038/s41590-020-0782-6
  contributor:
    fullname: Peng
– volume: 586
  year: 2020
  ident: B26
  article-title: ChAdOx1 nCoV-19 vaccine prevents SARS-CoV-2 pneumonia in rhesus macaques
  publication-title: Nature.
  doi: 10.1038/s41586-020-2608-y
  contributor:
    fullname: van Doremalen
– volume: 23
  year: 2005
  ident: B16
  article-title: The development of vaccines against SARS corona virus in mice and SCID-PBL/hu mice
  publication-title: Vaccine
  doi: 10.1016/j.vaccine.2005.01.036
  contributor:
    fullname: Okada
SSID ssj0000493335
Score 2.432975
Snippet Successful vaccine efforts countering the COVID-19 pandemic are centralized around the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S)...
SourceID doaj
pubmedcentral
proquest
crossref
SourceType Open Website
Open Access Repository
Aggregation Database
StartPage 1025500
SubjectTerms hamster model
Immunology
intranasal vaccination
SARS-CoV-2
severe acute respiratory syndrome coronavirus 2
vesicular stomatitis virus
SummonAdditionalLinks – databaseName: DOAJ Directory of Open Access Journals
  dbid: DOA
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1NT9wwELUqpEq9IGiLWKDISNyqiHzYSXzkU1CptBKw4mb8MYYFYVbsLmr_fWfipdqcuHBNIsV5Y2fesz3PjO0ihQ4S82xW2ALoCDOZWS9kFqgKtAQpwdI85M_z-vRK_LiW1wtHfdGesGQPnIDbE6pGimEMoBIRAlrMYFUoc--gVd4VqXQvLxfE1H3ivVVVyVQlgypM7YXR4-MM9WBZklsB8vK8l4k6w_4ey-zvkVxIOicrbHnOFvl-auUq-wDxM_uYzo_8-4Xd_E4uC4gtpzoRfvhreHaUFYrT9Co3fHgxzChPef5iHC2hc_iTdr7GW47Uj0_GowfgJnoeydgYE9sYuyfv3BtGcfKVXZ0cXx6eZvMjEzInhJxmCK2rG68KQMSCNVK4SoB0TVASTHA4-mzV2DpXKEyL2su2dI0z0lmifrmr1thSfIqwzri3FrWJAl-EWgRprCoNip8WKictxnHAvr_Cp8fJGUOjoiCwdQe2JrD1HOwBOyCE_z9JrtbdBYy1nsdavxXrAdt5jY_GUUBLGybC02yikYTJokY12w5Y0wtc7439O3F01_lp0z8IWd3GezRxk32iz6bsVoottjR9nsE3pC1Tu9310H-GxewG
  priority: 102
  providerName: Directory of Open Access Journals
– databaseName: Scholars Portal Open Access Journals
  dbid: M48
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV1Lb9QwELaqVkhcqpaHWEqRkbhVhnViO_GhqtpCVZBakGBXvRnbsdsF6l32Ue3-e2aSLCISHHrMQ3l8M5P5PseeIeQ1UOgoIc8y7njAFmaSuUpIFnEVaBakDA7HIS8u1flAfLySVxtk3e6oBXD2T2mH_aQG059vlr9WRxDwh6g4Id--jaPb2wVIvSzDQgRAuUHCb2WQGXGK10VL9783bDjP656bXCnBwJdFs47mP5fp5Kq6pH-Hh3ZnUf6Vls52yHbLJ-lx4wC7ZCOkR-RB02Fy9Zh8-9zUYQD0Ka4koaefhh_eMa4pDsBSS4dfhgwzWUXvrMef7DQsm7mx6ZoCOaSzyehHoDZVNGHpY0h9E3BgWtd3GKXZEzI4e__19Jy1TRWYF0LOGYDvVVFpHqwN0VkpfC6C9EXUMtjoIT5dXjjV1yBduapkmfnCW-kdksO-z5-SzTRO4RmhlXOgXnSoeFQiSut0ZkEelSH30oGle-RgDZ-ZNLUzDGgOBNvUYBsE27Rg98gJIvznTKx7Xe8YT69NG0ZGaAWEE54cdKkQoQQ-k8esDzYodeW57pFXa_sYiBP8-WFTGC9mBmia5Ar0btkjRcdwnTt2j6TRTV1xG79SwPue3-uF9shD3MREl4kXZHM-XYR9YDBz97J2y99cfe5n
  priority: 102
  providerName: Scholars Portal
Title Protection from COVID-19 with a VSV-based vaccine expressing the spike and nucleocapsid proteins
URI https://search.proquest.com/docview/2735167218
https://pubmed.ncbi.nlm.nih.gov/PMC9638159
https://doaj.org/article/496932aae84044e88193f20dce89dc19
Volume 13
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lj9MwELZ2V0LigniK8lgZiRvKtk48SXyEwrIgFVaCrXoztmPvBqhbbVsE_54ZJ0WbKxcf8lCcmbHm--zxZ8ZeIoQOgHk2E1Z4OsIMMttIyALtAs09gLc0Dzn7VJ5dyI8LWBww2O-FSUX7zrYn8efyJLZXqbZyvXTjfZ3Y-Hw2paDBNDw-ZIeYfm9Q9O8d5C2KAroNMkjA1Di0y-UOqWCek1ABQvLJIAklrf4BwByWR97IN6d32Z0eKPLXXYfusQMf77Nb3dGRfx6wb-edwAKaldMWET79PP_wNhOK08wqN3z-ZZ5Rimr4L-No9Zz7313Ra7zkiPr4Zt3-8NzEhkfSNMactsbI5Em4oY2bh-zi9N3X6VnWn5aQOSlhm6FVXVk1SnhjfLAGpCukB1cFBd4EhwPPFpUtJwo5qSgbqHNXOQPOEuqbuOIRO4qr6B8z3liLtET5RoRSBjBW5QZ5T-0LBxZdOGKv9ubT604UQyOZIGPrZGxNxta9sUfsDVn435MkaJ0urK4vde9WLVWJSBJ7joRTSl8jUClCPkEf1KpxQo3Yi71_NA4AWtUw0a92G434C0SJRLYesWrguMEXh3cwspKUdh9JT_77zafsNv0rZbNcPmNH2-udf44wZWuPE73H9v1CYDuT9XEK1L_qse4o
link.rule.ids 230,314,727,780,784,864,885,2102,24318,27924,27925,53791,53793
linkProvider National Library of Medicine
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3db9MwELfGEIIXxKfW8WUk3lDWOvYl8SMUpg7WMYmt2puxHWcEVrdaWwT_PXdJipZXXvOhOL87634_--7M2Buk0BVgnE2EE4GOMIPElQqSiqpA0wAQHK1DTk-yybn6dAEXOwy2tTBN0r539UG8mh_E-nuTW7mc--E2T2x4Oh2T02AYHt5it0HmWtwQ6T9a0iulhLZEBiWYHlb1fL5BMZim1KoASfmoF4aabv09itlPkLwRcQ4fsPsdVeTv2iE9ZDshPmJ32sMj_zxm307bFgsILKciET7-Mjv6kAjNaW2VWz77OksoSJX8l_W0f87D7zbtNV5y5H18tax_Bm5jySN1NcaotkTf5E3rhjqunrDzw49n40nSnZeQeKVgnSCuPstLLYK1oXIWlJcqgM8rDcFWHqeek7nLRhpVqchKKFKfewveEe8befmU7cZFDHuMl86hMNGhFFWmKrBOpxaVTxGkB4dGHLC3W_jMsm2LYVBOENimAdsQ2KYDe8DeE8L_nqSW1s2FxfWl6QxrlM6QS-LIUXIqFQqkKrJKR2iDQpde6AF7vbWPwSlA-xo2hsVmZZCBgchQyhYDlvcM1_ti_w76VtNMu_Ol_f9-8xW7OzmbHpvjo5PPz9g9-m-Kbal6znbX15vwAknL2r1sXPQvy33uvQ
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9QwELagCMQF8VS3vIzEDaXZxHYSH2HLqgVaVoKuejN-lkDXG3V3Efx7ZpIs2ly55qE434w132ePZwh5DRQ6CIizSWYyjy3MRGIcF0nAU6C5F8IbXIc8PSuOz_mHC3Gx0-qrTdq3pj6MV4vDWH9vcyubhU23eWLp7HSCTgNhOG1cSG-SW4KBk-0I9R8d8WWMie6YDMgwmYZ6sdiAIMxzLFcAxHw8CEVtxf4BzRwmSe5Enel9cq-ni_RtN6wH5IaPD8ntroHkn0fk26wrswDgUjwoQief5ydHSSYprq9STedf5gkGKkd_aYt76NT_7lJf4yUF7kdXTf3TUx0djVjZGCJbA_5J2_INdVw9JufT918nx0nfMyGxnIt1AtjaonQy81r7YLTglnEvbBmk8DpYmH6GlaYYS1CmWeFEldvSamENcr-xZU_IXlxGv0-oMwbEifQuCwUPQhuZa1A_lWdWGDDkiLzZwqearjSGAkmBYKsWbIVgqx7sEXmHCP97EstatxeW15eqN67isgA-CSMH2cm5r4CusJCPwQaVdDaTI_Jqax8F0wD3NnT0y81KAQsTWQFythqRcmC4wReHd8C_2oLavT8d_PebL8md2dFUfTo5-_iU3MXfxvCW82dkb3298c-Bt6zNi9ZD_wIvku_Q
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Protection+from+COVID-19+with+a+VSV-based+vaccine+expressing+the+spike+and+nucleocapsid+proteins&rft.jtitle=Frontiers+in+immunology&rft.au=O%E2%80%99Donnell%2C+Kyle+L.&rft.au=Gourdine%2C+Tylisha&rft.au=Fletcher%2C+Paige&rft.au=Clancy%2C+Chad+S.&rft.date=2022-10-24&rft.issn=1664-3224&rft.eissn=1664-3224&rft.volume=13&rft_id=info:doi/10.3389%2Ffimmu.2022.1025500&rft.externalDBID=n%2Fa&rft.externalDocID=10_3389_fimmu_2022_1025500
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1664-3224&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1664-3224&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1664-3224&client=summon