Synthesis of the Carbohydrate Moiety of Glycoproteins from the Parasite Echinococcus granulosus and Their Antigenicity against Human Sera

• Published in: Molecules (Basel, Switzerland) Vol. 26; no. 18; p. 5652
• Main Authors: Hada, Noriyasu, Morita, Tokio, Ueda, Takashi, Masuda, Kazuki, Nakane, Hiromi, Ogane, Mami, Yamano, Kimiaki, Schweizer, Frank, Kiuchi, Fumiyuki
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 17.09.2021; MDPI
• Subjects: Animals; Antigens; Antigens, Helminth - immunology; biotin probe; Carbohydrates; Carbohydrates - biosynthesis; Carbohydrates - chemistry; Chromatography; Echinococcus granulosus; Echinococcus granulosus - metabolism; Enzyme-Linked Immunosorbent Assay; glycoprotein; Glycoproteins; Glycoproteins - chemistry; host-parasite interaction; Humans; Parasites; Parasites - metabolism; Parasitic diseases; Serum - immunology; Spectrum analysis; stereocontrolled synthesis; host-parasite interaction; stereocontrolled synthesis; glycoprotein; biotin probe; Echinococcus granulosus

Stereocontrolled syntheses of biotin-labeled oligosaccharide portions containing the carbohydrate moiety of glycoprotein from Echinococcus granulosus have been accomplished. Trisaccharide Galβ1-3Galβ1-3GalNAcα1-R (A), tetrasaccharide Galα1-4Galβ1-3Galβ1-3GalNAcα1-R (B), and pentasaccharide Galα1-4Ga...