Establishing a Highly Accurate Circulating Tumor Cell Image Recognition System for Human Lung Cancer by Pre-Training on Lung Cancer Cell Lines

• Published in: Cancers Vol. 17; no. 14; p. 2289
• Main Authors: Matsumiya, Hiroki, Terabayashi, Kenji, Kishi, Yusuke, Yoshino, Yuki, Mori, Masataka, Kanayama, Masatoshi, Oyama, Rintaro, Nemoto, Yukiko, Nishizawa, Natsumasa, Honda, Yohei, Kuwata, Taiji, Takenaka, Masaru, Chikaishi, Yasuhiro, Yoneda, Kazue, Kuroda, Koji, Ohnaga, Takashi, Sasaki, Tohru, Tanaka, Fumihiro
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 09.07.2025; MDPI
• Subjects: Accuracy; Algorithms; Antibodies; Artificial intelligence; Automation; Care and treatment; Cells; Classification; Datasets; Deep learning; Development and progression; Diagnosis; Enumeration; Health aspects; Lung cancer; Machine learning; Medical imaging; Medical personnel; Medical research; Mesothelioma; Metastasis; Microfluidics; Mutation; Neural networks; Risk factors; Software; Statistical analysis; Testing; Training; Transfer learning; Tumor cell lines; Tumor cells; Tumors; Viral antibodies; Japan; United States > US; lung cancer; tumor cell; transfer learning; cell lines; artificial intelligence
• ISSN: 2072-6694; 2072-6694
• DOI: 10.3390/cancers17142289

Background/Objectives: Circulating tumor cells (CTCs) are important biomarkers for predicting prognosis and evaluating treatment efficacy in cancer. We developed the “CTC-Chip” system based on microfluidics, enabling highly sensitive CTC detection and prognostic assessment in lung cancer and malignant pleural mesothelioma. However, the final identification and enumeration of CTCs require manual intervention, which is time-consuming, prone to human error, and necessitates the involvement of experienced medical professionals. Medical image recognition using machine learning can reduce workload and improve automation. However, CTCs are rare in clinical samples, limiting the training data available to construct a robust CTC image recognition system. In this study, we established a highly accurate artificial intelligence-based CTC recognition system by pre-training convolutional neural networks using images from lung cancer cell lines. Methods: We performed transfer learning of convolutional neural networks. Initially, the models were pre-trained using images obtained from lung cancer cell lines. The model’s accuracy was improved by training with a limited number of clinical CTC images. Results: Transfer learning significantly improved the CTC classification accuracy to an average of 99.51%, compared to 96.96% for a model trained solely on pre-trained cell lines (p < 0.05). This approach showed notable efficacy when clinical training images were limited, achieving statistically significant accuracy improvements with as few as 17 clinical CTC images (p < 0.05). Conclusions: Overall, our findings demonstrate that pre-training with cancer cell lines enables rapid and highly accurate automated CTC recognition even with limited clinical data, significantly enhancing clinical applicability and potential utility across diverse cancer diagnostic workflows.